Muro A, Rodriguez-Medina J R, Hillyer G V
Department of Pathology, University of Puerto Rico School of Medicine, San Juan.
Am J Trop Med Hyg. 1993 Mar;48(3):457-63. doi: 10.4269/ajtmh.1993.48.457.
A lambda gt11 Fasciola hepatica cDNA library was previously constructed from poly(A)+ RNA extracted from adult worms. A cDNA encoding F. hepatica glutathione S-transferase (FhGST) (GenBank Accession Number M93434) was cloned by screening the library with a rabbit anti-FhGST antiserum. The FhGSTs are suspected to be early developmentally expressed-proteins that act as potent immunogens and may be useful as candidate vaccines against the parasite. The cDNA was sequenced and contains 627 translated bases encoding a 24,211-Dalton polypeptide with 209 amino acids and an isoelectric point (pI) of 5.324. This polypeptide has significant homology with 26-kD GSTs from Schistosoma mansoni (57.42%) and S. japonicum (57.14%). In addition, we have identified the predicted T cell epitopes in the FhGST protein molecule.
一个λgt11肝片吸虫cDNA文库先前是从成年虫体提取的聚腺苷酸加尾RNA构建而成。通过用兔抗肝片吸虫谷胱甘肽S-转移酶（FhGST）抗血清筛选该文库，克隆了一个编码肝片吸虫谷胱甘肽S-转移酶（FhGST）（GenBank登录号M93434）的cDNA。FhGST被怀疑是早期发育表达的蛋白质，作为有效的免疫原，可能用作抗该寄生虫的候选疫苗。对该cDNA进行了测序，其包含627个编码209个氨基酸、等电点（pI）为5.324、分子量为24211道尔顿的多肽的翻译碱基。该多肽与曼氏血吸虫（57.42%）和日本血吸虫（57.14%）的26-kD GST具有显著同源性。此外，我们还鉴定了FhGST蛋白分子中预测的T细胞表位。
