通过淘选随机肽展示文库鉴定出的透明质酸结合基序。

Hyaluronan-binding motif identified by panning a random peptide display library.

作者信息

Amemiya Kana, Nakatani Tatsuya, Saito Akio, Suzuki Atsuo, Munakata Hiroshi

机构信息

Department of Biochemistry, Kinki University School of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan.

出版信息

Biochim Biophys Acta. 2005 Jun 20;1724(1-2):94-9. doi: 10.1016/j.bbagen.2005.04.029.

DOI:10.1016/j.bbagen.2005.04.029

PMID:15921857

Abstract

The glycosaminoglycan hyaluronan (HA) is involved in a variety of functions such as cell migration, adhesion, activation of intracellular signaling, metastasis, inflammation and wound repair. These functions of HA are mediated via HA-binding proteins (HABPs). To derive details of the HA-binding site in HABPs, here, we panned a random peptide display library expressed on the E. coli flagellin protein using HA-coated plates. Using this random peptide display library, 40 positive clones were obtained and the nucleotide sequences were determined. As a result, an Arg-Arg sequence, in addition to the known B-X7-B motif, was found to bind to HA. A binding experiment using the IAsys resonant mirror biosensor verified that a peptide containing an Arg-Arg sequence binds to HA.

摘要

糖胺聚糖透明质酸（HA）参与多种功能，如细胞迁移、黏附、细胞内信号激活、转移、炎症和伤口修复。HA的这些功能是通过HA结合蛋白（HABPs）介导的。为了获取HABPs中HA结合位点的详细信息，在此，我们使用HA包被的平板筛选了在大肠杆菌鞭毛蛋白上表达的随机肽展示文库。利用该随机肽展示文库，获得了40个阳性克隆并测定了核苷酸序列。结果发现，除了已知的B-X7-B基序外，一个精氨酸-精氨酸序列也能与HA结合。使用IAsys共振镜生物传感器进行的结合实验证实，含有精氨酸-精氨酸序列的肽能与HA结合。