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基于合成肽的荧光探针用于透明质酸的开启检测设计。

Design of synthetic peptide-based fluorescence probes for turn-on detection of hyaluronan.

机构信息

Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan.

出版信息

Anal Sci. 2024 Apr;40(4):609-614. doi: 10.1007/s44211-023-00491-6. Epub 2024 Jan 12.

DOI:10.1007/s44211-023-00491-6
PMID:38214835
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10961276/
Abstract

Herein, we designed and examined a series of fluorescent peptide-based probes for turn-on detection of hyaluronan (HA), a member of the glycosaminoglycan family. We utilized two kinds of synthetic HA-binding peptides as the binding unit for HA, and each peptide was coupled with three kinds of environment-sensitive fluorophores as the signaling unit. From the examination of the peptides, fluorophores, and the position and number of fluorophore modification, we found that X7 peptide (RYPISRPRKR) labelled with an aggregation-induced emission (AIE) fluorogen, tetraphenylethene (TPE), at the N-terminal (named TPE-X7) did function as a light-up probe for HA. The response of TPE-X7 was highly selective to higher molecular weight HA in comparison with lower ones, having the possible potential for the analysis of HA size. TPE-X7 was also applicable to the quantification of HA in synovial fluids.

摘要

在这里，我们设计并研究了一系列基于荧光肽的探针，用于透明质酸（HA）的开启检测，HA 是糖胺聚糖家族的一员。我们利用两种合成的 HA 结合肽作为 HA 的结合单元，每个肽都与三种环境敏感的荧光团偶联作为信号单元。通过对肽、荧光团以及荧光团修饰的位置和数量的研究，我们发现，在 N 端标记具有聚集诱导发射（AIE）特性的荧光团四苯乙烯（TPE）的 X7 肽（RYPISRPRKR）（命名为 TPE-X7）可作为 HA 的点亮探针。与低分子量的 HA 相比，TPE-X7 对高分子量的 HA 的响应具有高度选择性，具有分析 HA 大小的潜在可能性。TPE-X7 也可用于滑液中 HA 的定量分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e2d/10961276/1ebf94adaa39/44211_2023_491_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e2d/10961276/d5523e5d4fcd/44211_2023_491_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e2d/10961276/784e483f9347/44211_2023_491_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e2d/10961276/645b64cff52e/44211_2023_491_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e2d/10961276/bdf0ea973ec9/44211_2023_491_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e2d/10961276/1ebf94adaa39/44211_2023_491_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e2d/10961276/d5523e5d4fcd/44211_2023_491_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e2d/10961276/784e483f9347/44211_2023_491_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e2d/10961276/645b64cff52e/44211_2023_491_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e2d/10961276/bdf0ea973ec9/44211_2023_491_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e2d/10961276/1ebf94adaa39/44211_2023_491_Fig5_HTML.jpg

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