Liu Lu-Ning, Chen Xiu-Lan, Zhang Yu-Zhong, Zhou Bai-Cheng
State Key Lab of Microbial Technology, Shandong University, Jinan 250100, PR China.
Biochim Biophys Acta. 2005 Jun 30;1708(2):133-42. doi: 10.1016/j.bbabio.2005.04.001.
Cyanobacteria and red algae have intricate light-harvesting systems comprised of phycobilisomes that are attached to the outer side of the thylakoid membrane. The phycobilisomes absorb light in the wavelength range of 500-650 nm and transfer energy to the chlorophyll for photosynthesis. Phycobilisomes, which biochemically consist of phycobiliproteins and linker polypeptides, are particularly wonderful subjects for the detailed analysis of structure and function due to their spectral properties and their various components affected by growth conditions. The linker polypeptides are believed to mediate both the assembly of phycobiliproteins into the highly ordered arrays in the phycobilisomes and the interactions between the phycobilisomes and the thylakoid membrane. Functionally, they have been reported to improve energy migration by regulating the spectral characteristics of colored phycobiliproteins. In this review, the progress regarding linker polypeptides research, including separation approaches, structures and interactions with phycobiliproteins, as well as their functions in the phycobilisomes, is presented. In addition, some problems with previous work on linkers are also discussed.
蓝细菌和红藻拥有复杂的光捕获系统，该系统由附着在类囊体膜外侧的藻胆体组成。藻胆体吸收波长在500 - 650纳米范围内的光，并将能量传递给叶绿素用于光合作用。藻胆体在生物化学上由藻胆蛋白和连接多肽组成，由于其光谱特性以及其各种成分受生长条件影响，它们是进行结构和功能详细分析的绝佳对象。连接多肽被认为既介导藻胆蛋白组装成藻胆体中的高度有序阵列，又介导藻胆体与类囊体膜之间的相互作用。在功能上，据报道它们通过调节有色藻胆蛋白的光谱特性来改善能量迁移。在这篇综述中，介绍了关于连接多肽研究的进展，包括分离方法、结构以及与藻胆蛋白的相互作用，还有它们在藻胆体中的功能。此外，还讨论了先前关于连接体研究工作中存在的一些问题。
