芽孢杆菌中一个假定的新型α/β水解酶孤儿家族。

A putative novel alpha/beta hydrolase ORFan family in Bacillus.

作者信息

Siew Naomi, Saini Harpreet Kaur, Fischer Daniel

机构信息

Department of Chemistry, Ben Gurion University, Beer-Sheva 84105, Israel.

出版信息

FEBS Lett. 2005 Jun 6;579(14):3175-82. doi: 10.1016/j.febslet.2005.04.030.

DOI:10.1016/j.febslet.2005.04.030

PMID:15922334

Abstract

A large number of sequences in each newly sequenced genome correspond to lineage and species-specific proteins, also known as ORFans. Amongst these ORFans, a large number are sequences with unknown structures and functions. We have identified a family of sequences, annotated as hypothetical proteins, which are specific to Bacillus and have carried out a computational study aimed at characterizing this family. Fold-recognition methods predict that these sequences belong to the alpha/beta hydrolase fold. We suggest possible catalytic triads for the ORFans and propose a hypothesis regarding the possible families within the alpha/beta hydrolase superfamily to which they may belong.

摘要

每个新测序的基因组中有大量序列对应于谱系和物种特异性蛋白质，也称为孤儿基因（ORFans）。在这些孤儿基因中，大量是结构和功能未知的序列。我们鉴定了一个被注释为假设蛋白质的序列家族，该家族是芽孢杆菌属特有的，并进行了一项旨在表征该家族的计算研究。折叠识别方法预测这些序列属于α/β水解酶折叠。我们为孤儿基因提出了可能的催化三联体，并提出了一个关于它们可能所属的α/β水解酶超家族中可能家族的假设。