Angus Robert A, Stanko Jason, Jenkins Ronald L, Watson R Douglas
Department of Biology, University of Alabama at Birmingham, 378 Campbell Hall, 1300 University Boulevard, Birmingham, AL 35294-1170, USA.
Comp Biochem Physiol C Toxicol Pharmacol. 2005 Mar-Apr;140(3-4):330-9. doi: 10.1016/j.cca.2005.03.008.
Juvenile male western mosquitofish (Gambusia affinis) were exposed to different concentrations of 17alpha-ethynyl estradiol (EE2) in the diet during the period of sexual maturation. A clear inhibiting influence of EE2 on sexual development was apparent. The proportion of males in each treatment group that failed to complete gonopodial development during the 150-day observation period increased significantly with EE2 concentration. There were significant nonlinear trends toward shorter gonopodia in groups exposed to higher EE2 concentrations. Vitellogenin (VTG) was detectable in the blood of all fish exposed to 1.0 or more micro/g food and the concentration increased dramatically with increasing EE2 exposure. A significant negative association was seen between EE2 concentration and spermatophore counts. This study has demonstrated deleterious effects of EE2 exposure on sexual maturation and several indirect measures of reproductive fitness. It supports the biological relevance of vitellogenin in the blood and reduced gonopodium length as biomarkers for estrogen exposure and endocrine disruption in mosquitofish.
在性成熟期间，将幼年雄性西部食蚊鱼（盖氏食蚊鱼）置于含有不同浓度17α-乙炔基雌二醇（EE2）的饲料中。EE2对性发育具有明显的抑制作用。在150天的观察期内，每个处理组中未能完成生殖足发育的雄性比例随EE2浓度的增加而显著上升。暴露于较高EE2浓度组的生殖足有显著的非线性缩短趋势。在所有暴露于1.0微克/克及以上饲料EE2的鱼类血液中均可检测到卵黄蛋白原（VTG），且其浓度随EE2暴露量的增加而急剧上升。EE2浓度与精包计数之间存在显著的负相关。本研究证明了EE2暴露对性成熟以及生殖适应性的几种间接指标具有有害影响。它支持了血液中卵黄蛋白原和生殖足长度缩短作为食蚊鱼雌激素暴露和内分泌干扰生物标志物的生物学相关性。
