Aoyama-Kondo T, Yoshida T, Tsobe K, Nakayama A, Asai J, Oka T, Nakashima I
Department of Immunology, Nagoya University School of Medicine, Japan.
Immunobiology. 1992 Apr;184(4-5):372-83. doi: 10.1016/s0171-2985(11)80594-2.
We studied the function of submandibular lymph nodes (MLN) in the oral mucosa immune system as compared with that of inguinal lymph nodes (ILN) in the cutaneous one. Primary IgM, IgG and IgA antibody responses in MLN to sheep red blood cells (SRBC) as a model antigen given submucosally occurred more extensively than those in ILN to the antigen injected subcutaneously. Particularly, definite IgA synthesis was seen in MLN but not in ILN. This IgA synthesis was shown to be originated locally in oral submucosal lymphoid tissue or MLN but not in gut-associated lymphoid tissue (GALT). This suggested that the oral mucosal tissue including MLN acts like Peyer's patches in GALT for IgA synthesis. When mice were administered with SRBC and bacterial lipopolysaccharide (LPS) submucosally, the adjuvant effect of LPS was only observed on the capacity of MLN cells for secondary antibody response in vitro. This contrasted to the marked augmentation by LPS of both the primary antibody response in ILN and capacity for in vitro secondary antibody response of ILN cells of mice given SRBC and LPS subcutaneously. The radioactivities detected in the local lymph nodes and other tissues of mice given 51Cr labeled SRBC submucosally or subcutaneously were comparable with each other. MLN, however, contained more Ig+/B220+ B cells and less Thy1+/Ly-1+ T cells than ILN did, and the L3T4/Ly-2 ratio of T cell subpopulations in MLN was lower than that in ILN. Partially corresponding to this observation, the B cell-dependent area was developed more extensively in MLN than in ILN. This difference in cellular composition and organization might in part explain the reason why MLN and ILN display distinct modes of response and sensitivity to the action of LPS.
我们研究了下颌下淋巴结（MLN）在口腔黏膜免疫系统中的功能，并与腹股沟淋巴结（ILN）在皮肤免疫系统中的功能进行了比较。以绵羊红细胞（SRBC）作为模型抗原，经黏膜下给予后，MLN中针对该抗原的原发性IgM、IgG和IgA抗体反应比经皮下注射抗原后ILN中的反应更为广泛。特别是，在MLN中可见明确的IgA合成，而在ILN中则未见到。这种IgA合成被证明起源于口腔黏膜下淋巴组织或MLN局部，而非肠道相关淋巴组织（GALT）。这表明包括MLN在内的口腔黏膜组织在IgA合成方面的作用类似于GALT中的派尔集合淋巴结。当给小鼠经黏膜下注射SRBC和细菌脂多糖（LPS）时，仅在体外观察到LPS对MLN细胞二次抗体反应能力的佐剂作用。这与皮下注射SRBC和LPS的小鼠中，LPS显著增强ILN中的原发性抗体反应以及ILN细胞体外二次抗体反应能力形成对比。经黏膜下或皮下给予51Cr标记的SRBC后，在小鼠局部淋巴结和其他组织中检测到的放射性彼此相当。然而，MLN中Ig+/B220+ B细胞比ILN多，Thy1+/Ly-1+ T细胞比ILN少，并且MLN中T细胞亚群的L3T4/Ly-2比值低于ILN。部分与此观察结果相对应，MLN中B细胞依赖区的发育比ILN更广泛。细胞组成和组织结构的这种差异可能部分解释了MLN和ILN对LPS作用表现出不同反应模式和敏感性的原因。
