Rogers Phillip H, Michel Eric, Bauer Carl A, Vanderet Stephen, Hansen Daniel, Roberts Bradley K, Calvez Antoine, Crews Jackson B, Lau Kwok O, Wood Alistair, Pine David J, Schwartz Peter V
Physics Department, California Polytechnic State University, San Luis Obispo, California 93407, USA.
Langmuir. 2005 Jun 7;21(12):5562-9. doi: 10.1021/la046790y.
The directed three-dimensional self-assembly of microstructures and nanostructures through the selective hybridization of DNA is the focus of great interest toward the fabrication of new materials. Single-stranded DNA is covalently attached to polystyrene latex microspheres. Single-stranded DNA can function as a sequence-selective Velcro by only bonding to another strand of DNA that has a complementary sequence. The attachment of the DNA increases the charge stabilization of the microspheres and allows controllable aggregation of microspheres by hybridization of complementary DNA sequences. In a mixture of microspheres derivatized with different sequences of DNA, microspheres with complementary DNA form aggregates, while microspheres with noncomplementary sequences remain suspended. The process is reversible by heating, with a characteristic "aggregate dissociation temperature" that is predictably dependent on salt concentration, and the evolution of aggregate dissociation with temperature is observed with optical microscopy.
通过DNA的选择性杂交实现微结构和纳米结构的定向三维自组装，是制备新材料的研究热点。单链DNA共价连接到聚苯乙烯乳胶微球上。单链DNA仅能与具有互补序列的另一条DNA链结合，从而起到序列选择性“魔术贴”的作用。DNA的附着增加了微球的电荷稳定性，并通过互补DNA序列的杂交实现微球的可控聚集。在衍生有不同DNA序列的微球混合物中，具有互补DNA的微球形成聚集体，而具有非互补序列的微球则保持悬浮状态。该过程通过加热可逆，具有可预测地依赖于盐浓度的特征“聚集体解离温度”，并且通过光学显微镜观察聚集体解离随温度的变化。
