Damase Tulsi R, Stephens Daniel, Spencer Adam, Allen Peter B
Department of Chemistry, University of Idaho, Moscow, Idaho, USA.
J Biol Methods. 2015;2(3):e24. doi: 10.14440/jbm.2015.72.
A set of instruments and specialized equipment is necessary to equip a laboratory to work with DNA. Reducing the barrier to entry for DNA manipulation should enable and encourage new labs to enter the field. We present three examples of open source/DIY technology with significantly reduced costs relative to commercial equipment. This includes a gel scanner, a horizontal PAGE gel mold, and a homogenizer for generating DNA-coated particles. The overall cost savings obtained by using open source/DIY equipment was between 50 and 90%.
配备一个用于处理DNA的实验室需要一套仪器和专业设备。降低DNA操作的准入门槛应能促使并鼓励新实验室进入该领域。我们展示了三个开源/自制技术的例子,其成本相对于商业设备大幅降低。这包括一台凝胶扫描仪、一个水平PAGE凝胶模具以及一个用于生成DNA包被颗粒的匀浆器。使用开源/自制设备所实现的总体成本节约在50%至90%之间。