Eichlerová Ivana, Homolka Ladislav, Lisá Ludmila, Nerud Frantisek
Institute of Microbiology AS CR, Vídenská 1083, 142 20 Prague 4, Czech Republic.
Chemosphere. 2005 Jul;60(3):398-404. doi: 10.1016/j.chemosphere.2004.12.036.
Thirty different white rot strains were screened for Orange G and Remazol Brilliant Blue R (RBBR) decolorization on agar plates. Three promising strains, Dichomitus squalens, Ischnoderma resinosum and Pleurotus calyptratus, selected on the basis of this screening, were used for decolorization study in liquid media. All three strains efficiently decolorized both Orange G and RBBR, but they differed in decolorization capacity depending on cultivation conditions and ligninolytic enzyme production. Two different decolorization patterns were found in these strains: Orange G decolorization in I. resinosum and P. calyptratus was caused mainly by laccase, while RBBR decolorization was effected by manganese peroxidase (MnP); in D. squalens laccase and MnP cooperated in the decolorization processes.
在琼脂平板上对30种不同的白腐菌菌株进行了橙黄G和活性艳蓝R（RBBR）脱色筛选。基于此筛选选出的3株有潜力的菌株，即鳞皮扇菇、树脂硬皮马勃和杯状侧耳，用于液体培养基中的脱色研究。所有这3株菌株都能有效地使橙黄G和RBBR脱色，但根据培养条件和木质素分解酶的产生情况，它们的脱色能力有所不同。在这些菌株中发现了两种不同的脱色模式：树脂硬皮马勃和杯状侧耳中橙黄G的脱色主要由漆酶引起，而RBBR的脱色则由锰过氧化物酶（MnP）实现；在鳞皮扇菇中，漆酶和MnP在脱色过程中协同作用。
