Susla Martin, Novotný Cenek, Svobodová Katerina
Laboratory of Experimental Mycology, Institute of Microbiology AS CR, Vídenská 1083, 142 20 Prague 4, Czech Republic.
Bioresour Technol. 2007 Aug;98(11):2109-15. doi: 10.1016/j.biortech.2006.08.007. Epub 2006 Oct 10.
The study focuses on the production of ligninolytic enzymes and dye degradation capacity of Dichomitus squalens immobilized on polyurethane foam (PUF) or pine wood (PW) in a fixed bed reactor at a laboratory scale (working volume of 0.6l). Immobilization of fungal cultures on pine wood improved eminently laccase production in comparison to the liquid cultures. Immobilized D. squalens was able to decolorize an anthraquinone dye Remazol Brilliant Blue R and an azo dye Reactive Orange 16, however, only a limited decolorization of Copper(II)phthalocyanine dye was observed in both types of reactor cultures. The involvement of a laccase activity in dye decolorization was suggested. Further, two different chromatographical forms of laccases, Lc1 and Lc2, were isolated from PW cultures of D. squalens using a fast, two step FPLC method. Enzymes revealed identical molecular masses of 68 kDa (estimated by SDS-PAGE) and similar pI's, however, they differed in their catalytic properties such as pH dependence of the activity and ABTS oxidation rates. In this study, we demonstrated different dye decolorization capacities of Lc1 and Lc2 as well.
该研究聚焦于在实验室规模的固定床反应器（工作体积0.6升）中，固定在聚氨酯泡沫（PUF）或松木（PW）上的黄白卧孔菌产生木质素分解酶的情况及其染料降解能力。与液体培养相比，将真菌培养物固定在松木上显著提高了漆酶的产量。固定化的黄白卧孔菌能够使蒽醌染料雷马素亮蓝R和偶氮染料活性橙16脱色，然而，在两种类型的反应器培养物中，仅观察到铜（II）酞菁染料有有限的脱色。研究表明漆酶活性参与了染料脱色过程。此外，使用快速的两步快速蛋白质液相色谱（FPLC）方法，从黄白卧孔菌的PW培养物中分离出两种不同色谱形式的漆酶，Lc1和Lc2。这些酶显示出相同的68 kDa分子量（通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）估计）和相似的等电点，但它们在催化特性上有所不同，如活性的pH依赖性和2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）氧化速率。在本研究中，我们还证明了Lc1和Lc2具有不同的染料脱色能力。
