Zhang Peng, Li Fei, Yang Xiu-Wei
The State Key Laboratory of Natural and Biomimetic Drugs, and Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100083, People's Republic of China.
Biomed Chromatogr. 2008 Jul;22(7):758-62. doi: 10.1002/bmc.994.
A simple, sensitive and selective RP-HPLC method has been developed for quantification of nodakenin in rat plasma. Nodakenin in rat plasma was extracted with acetonitrile, which also acted as a deproteinization agent. Chromatographic separation of nodakenin was performed on an analytical Diamonsil ODS C18 column, with a mobile phase of MeOH-H2O (1:1, v/v) at a flow-rate of 1.0 mL/min, and UV detection was set at 330 nm. The calibration curve was linear over the range 0.2-12.0 microg/mL (R2 = 0.9995) in rat plasma. The lower limit of detection and quantification were 0.01 and 0.1 microg/mL, respectively, using the rat plasma sample. The extraction recoveries were 77.36 +/- 4.56, 82.89 +/- 1.84 and 81.66 +/- 2.49% at concentrations of 1.0, 5.0 and 10.0 microg/mL, respectively. The intra- and inter-day precision and accuracy were validated by relative standard deviation and relative error, which were in the ranges 5.07-5.83 and 3.95-6.29%, respectively. After i.v. administration to rats at a single dose of 40 mg/kg, the plasma concentration-time curve of nodakenin was best conformed to a two-compartment open model. This assay method has been successfully applied to the study of the pharmacokinetics of nodakenin in rats.
已开发出一种简单、灵敏且具选择性的反相高效液相色谱法用于定量大鼠血浆中的紫花前胡苷。大鼠血浆中的紫花前胡苷用乙腈萃取，乙腈同时作为蛋白沉淀剂。紫花前胡苷的色谱分离在Diamonsil ODS C18分析柱上进行，流动相为甲醇 - 水（1:1，v/v），流速为1.0 mL/min，紫外检测波长设定为330 nm。在大鼠血浆中，校准曲线在0.2 - 12.0 μg/mL范围内呈线性（R2 = 0.9995）。使用大鼠血浆样品时，检测限和定量下限分别为0.01和0.1 μg/mL。在浓度为1.0、5.0和10.0 μg/mL时，萃取回收率分别为77.36 ± 4.56%、82.89 ± 1.84%和81.66 ± 2.49%。日内和日间精密度及准确度通过相对标准偏差和相对误差进行验证，相对标准偏差范围为5.07 - 5.83%，相对误差范围为3.95 - 6.29%。以40 mg/kg的单次剂量静脉注射给大鼠后，紫花前胡苷的血浆浓度 - 时间曲线最符合二室开放模型。该测定方法已成功应用于大鼠体内紫花前胡苷的药代动力学研究。
