使用超薄针进行基因表达，可实现精确移位且侵入性低。

Gene expression using an ultrathin needle enabling accurate displacement and low invasiveness.

作者信息

Han SungWoong, Nakamura Chikashi, Obataya Ikuo, Nakamura Noriyuki, Miyake Jun

机构信息

Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan.

出版信息

Biochem Biophys Res Commun. 2005 Jul 8;332(3):633-9. doi: 10.1016/j.bbrc.2005.04.059.

DOI:10.1016/j.bbrc.2005.04.059

PMID:15925564

Abstract

We have previously demonstrated a new cell manipulation technology by using an atomic force microscope (AFM) and ultrathin needles, named nanoneedles. The nanoneedle is an AFM tip etched by a focused ion beam (FIB) and is sharpened from 200 to 800 nm in diameter. In this study, we have evaluated the proper diameter of a needle required for insertion into human cells over a long period without causing cell death, and achieved highly efficient gene expression method for human cells using a nanoneedle and an AFM.

摘要

我们之前展示了一种利用原子力显微镜（AFM）和超薄针（称为纳米针）的新型细胞操作技术。纳米针是通过聚焦离子束（FIB）蚀刻的AFM探针，其直径从200纳米锐化到800纳米。在本研究中，我们评估了长时间插入人体细胞而不导致细胞死亡所需的合适针直径，并利用纳米针和AFM实现了高效的人体细胞基因表达方法。