Han Sung Woong, Nakamura Chikashi, Obataya Ikuo, Nakamura Noriyuki, Miyake Jun
Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan.
Biosens Bioelectron. 2005 Apr 15;20(10):2120-5. doi: 10.1016/j.bios.2004.08.023.
We developed a new low invasive cell manipulation and gene or molecule transfer system in a single living cell by using an atomic force microscope (AFM) and ultra thin needle, a nanoneedle. DNA was immobilized on the surface of the nanoneedle by covalent bonding and avidin-biotin affinity binding. Immobilization of DNA on the nanoneedle was confirmed by measuring the unbinding force between avidin and biotin. The DNA-immobilized nanoneedle was successfully inserted into HEK293 cells. Though TO-PRO-3 iodide staining experiments using confocal microscopy, we observed the immobilized DNA on the surface of the nanoneedle, which was retained after 10 times insertions to and evacuations from a living cell.
我们利用原子力显微镜（AFM）和超薄针（一种纳米针）开发了一种新型的低侵入性细胞操作以及在单个活细胞中进行基因或分子转移的系统。通过共价键合和抗生物素蛋白-生物素亲和结合将DNA固定在纳米针表面。通过测量抗生物素蛋白和生物素之间的解离力来确认DNA在纳米针上的固定。固定有DNA的纳米针成功插入HEK293细胞中。通过共聚焦显微镜进行的碘化丙锭染色实验，我们观察到纳米针表面固定的DNA，在向活细胞插入和抽出10次后仍保留在细胞中。
Zotero 插件