利用AgAper1调控元件驱动转基因蚊子中肠特异性表达和分泌外源蛋白。

Driving midgut-specific expression and secretion of a foreign protein in transgenic mosquitoes with AgAper1 regulatory elements.

作者信息

Abraham E G, Donnelly-Doman M, Fujioka H, Ghosh A, Moreira L, Jacobs-Lorena M

机构信息

Department of Molecular Microbiology and Immunology, John Hopkins Bloomberg School of Public Health, Malaria Research Institute, Baltimore, MD 21205, USA.

出版信息

Insect Mol Biol. 2005 Jun;14(3):271-9. doi: 10.1111/j.1365-2583.2004.00557.x.

DOI:10.1111/j.1365-2583.2004.00557.x

PMID:15926896

Abstract

The Anopheles gambiae adult peritrophic matrix protein 1 (AgAper1) regulatory elements were used to drive the expression of phospholipase A2 (PLA2), a protein known to disrupt malaria parasite development in mosquitoes. These AgAper1 regulatory elements were sufficient to promote the accumulation of PLA2 in midgut epithelial cells before a blood meal and its release into the lumen upon blood ingestion. Plasmodium berghei oocyst formation was reduced by approximately 80% (74-91% range) in transgenic mosquitoes. Blood-seeking behaviour and survival of AgAper1-PLA2 transgenic mosquitoes were comparable to sibling wild-type mosquitoes, while fertility was substantially lower. Ultrastructural studies suggest that decreased fitness is a consequence of internal damage to midgut epithelial cells.

摘要

冈比亚按蚊成虫围食膜蛋白1（AgAper1）调控元件被用于驱动磷脂酶A2（PLA2）的表达，磷脂酶A2是一种已知会干扰疟原虫在蚊子体内发育的蛋白质。这些AgAper1调控元件足以促进血餐之前PLA2在中肠上皮细胞中的积累，并在摄入血液后将其释放到肠腔中。在转基因蚊子中，伯氏疟原虫卵囊形成减少了约80%（范围为74%-91%）。寻找血液行为和AgAper1-PLA2转基因蚊子的存活率与同胞野生型蚊子相当，而繁殖力则显著较低。超微结构研究表明，健康状况下降是中肠上皮细胞内部损伤的结果。