Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Mar Bermejo 195, Col. Playa Palo de Santa Rita, La Paz, B.C.S. 23096, Mexico.
Insect Biochem Mol Biol. 2010 Oct;40(10):752-8. doi: 10.1016/j.ibmb.2010.07.011. Epub 2010 Aug 6.
The main vector for transmission of malaria in Mexico is the Anopheles albimanus mosquito. The midgut of disease-transmitting mosquitoes carries out a variety of functions that are related to blood feeding. We analyzed the midgut of A. albimanus infected with Plasmodium berghei (resistant mosquito) using a proteomic approach to identify putative short peptides that are enriched in the midgut after blood feeding. Mosquito midguts were analyzed by two-dimensional electrophoresis to determine the changes in protein profiles. We identified 21 spot proteins that are differentially expressed in the blood of mosquitoes during the immune challenge. Molecular weight of the spots varied from 13 to 36 kDa, with a broad isoelectric point range of 3.92-8.90. We identified the differentially expressed proteins using mass spectrometry and constructed a proteomic data base of the A. albimanus midgut with diverse functions, some of them proteins with digestive and immunologic functions. Identification of these proteins may have important implications for understanding the blood meal digestion process, as well as developing novel vector control strategies and understanding parasite vector interactions.
在墨西哥，疟疾的主要传播媒介是致倦库蚊。传播疾病的蚊子的中肠执行多种与吸血有关的功能。我们使用蛋白质组学方法分析了感染伯氏疟原虫（抗蚊）的致倦库蚊的中肠，以鉴定在吸血后中肠中富集的推定短肽。通过二维电泳分析蚊子的中肠，以确定在免疫挑战期间蚊子血液中蛋白质图谱的变化。斑点蛋白的分子量变化范围为 13 至 36 kDa，等电点范围很宽，为 3.92-8.90。我们使用质谱法鉴定了差异表达蛋白，并构建了具有多种功能的致倦库蚊中肠蛋白质组数据库，其中一些蛋白具有消化和免疫功能。这些蛋白质的鉴定可能对理解血液消化过程以及开发新的媒介控制策略和理解寄生虫媒介相互作用具有重要意义。
