Darniot Magali, Petrella Tony, Aho Serge, Pothier Pierre, Manoha Catherine
Laboratoire de Virologie, CHU Dijon, Bld Marechal de Lattre de Tassigny, 21079 Dijon Cedex, France.
Vaccine. 2005 Aug 22;23(36):4473-80. doi: 10.1016/j.vaccine.2005.04.027.
Human metapneumovirus (hMPV), a recently identified virus, causes upper and lower respiratory tract diseases. In this study, we show that BALB/c mice inoculated with hMPV exhibited significant morbidity on 1--2 days post-infection, when airway obstruction was found. Increased airway hyper-responsiveness to metacholine was found on day 4 concurrent with lung viral replication. Both IgG1 and IgG2a hMPV-specific antibodies were found in sera, while interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) were found in bronchoalveolar lavage. Lung histology showed parenchymal pneumonia and increased lymphocytic infiltration. We present here an animal model that may be helpful in studying hMPV pathogenesis and evaluating the effects of vaccines.
人偏肺病毒（hMPV）是一种最近发现的病毒，可引起上、下呼吸道疾病。在本研究中，我们发现接种hMPV的BALB/c小鼠在感染后1-2天出现明显发病，此时发现气道阻塞。在第4天发现对乙酰甲胆碱的气道高反应性增加，同时伴有肺部病毒复制。血清中发现了IgG1和IgG2a hMPV特异性抗体，而支气管肺泡灌洗中发现了干扰素-γ（IFN-γ）和白细胞介素-4（IL-4）。肺组织学显示实质性肺炎和淋巴细胞浸润增加。我们在此提出一种动物模型，可能有助于研究hMPV发病机制并评估疫苗的效果。
