Jabłońska Ewa, Puzewska Wioletta, Marcińczyk Magdalena, Grabowska Zyta, Jabłoński Jakob
Department of Immunology, Medical University, Białystok, Poland.
Arch Immunol Ther Exp (Warsz). 2005 Mar-Apr;53(2):175-9.
The tumor-polymorphonuclear neutrophil (PMN) relationship can be altered by the release of toxic molecules, such as nitric oxide (NO). The aim of the present study was to examine the expression of the inducible synthase of NO (iNOS)and NO production by human neutrophils of patients with oral cavity cancer. For comparison we performed similar examinations in autologous peripheral blood mononuclear cells (PBMCs).
MATERIAL/METHODS: PMNs and PBMCs were isolated from the whole blood of 27 patients with squamous cell carcinoma of the oral cavity. iNOS protein expression in these cells was detected by Western blot. Total nitrite as an indicator of NO concentrations in the culture supernatants and the serum of patients was measured using a colorimetric assay.
The PMNs of oral cavity cancer patients showed a significantly lower intensity of iNOS expression than those of healthy controls. The PBMCs of patients showed a more intensive expression of iNOS than the PMNs, but a lower intensity than the PBMCs of the controls. The expression of iNOS in rhIL-6 and rhIL-15-stimulated PMNs and PBMCs of patients increased in comparison with unstimulated cells. We observed lower productions of NO by PMNs and PBMCs of patients than those of the control group.
The results revealed that altered iNOS expression and NO production are more characteristic of PMNs than of PBMCs of patients with oral cavity cancer. Additionally, this study provided new information about IL-6 and IL-15 activity in a tumor-bearing host.
肿瘤与多形核中性粒细胞（PMN）之间的关系可因一氧化氮（NO）等毒性分子的释放而改变。本研究旨在检测口腔癌患者的人类中性粒细胞中诱导型一氧化氮合酶（iNOS）的表达及NO的产生。为作比较，我们对自体外周血单个核细胞（PBMC）进行了类似检测。
材料/方法：从27例口腔鳞状细胞癌患者的全血中分离出PMN和PBMC。通过蛋白质免疫印迹法检测这些细胞中iNOS蛋白的表达。使用比色法测定培养上清液和患者血清中作为NO浓度指标的总亚硝酸盐。
口腔癌患者的PMN中iNOS表达强度明显低于健康对照者。患者的PBMC中iNOS表达比PMN更强烈，但低于对照组的PBMC。与未受刺激的细胞相比，患者经重组人白细胞介素-6（rhIL-6）和重组人白细胞介素-15（rhIL-15）刺激的PMN和PBMC中iNOS表达增加。我们观察到患者的PMN和PBMC产生的NO低于对照组。
结果显示，iNOS表达改变和NO产生在口腔癌患者的PMN中比在PBMC中更具特征性。此外，本研究提供了有关荷瘤宿主中白细胞介素-6和白细胞介素-15活性的新信息。
