Qu J, Thomas K
Department of Obstetrics and Gynecology, University of Louvain, Brussels, Belgium.
J Clin Endocrinol Metab. 1992 Jun;74(6):1290-5. doi: 10.1210/jcem.74.6.1592873.
The changes in bioinhibin (B-inhibin) and immunoinhibin (I-inhibin) levels were studied in the serum of healthy term pregnant women by ovine pituitary cell culture and immunoenzymatic assay systems before and 24 h after delivery. In the maternal serum, a sharp decline in both B-inhibin and I-inhibin levels was observed within first 6 h after delivery. B-inhibin and I-inhibin in maternal serum were 3.45 +/- 0.25 and 3.77 +/- 0.43 U/mL, respectively, before labor and decreased by 35.82% and 38.89% 30 min after labor. One hour after delivery, B-inhibin and I-inhibin were reduced by 56.74% and 61.48%, respectively. After 6 h, B-inhibin and I-inhibin levels were lowered by 93.86% and 78.90%, respectively. Twenty-four hours later, both inhibins were nearly undetectable. In the retroplacental serum, B-inhibin and I-inhibin were 4.92 +/- 0.34 and 10.13 +/- 1.16 U/mL, respectively, i.e. 42.48% and 168.60% higher than the levels of B-inhibin (P less than 0.05) and I-inhibin (P less than 0.001) in maternal serum before delivery. In the umbilical cord serum, the B-inhibin concentration was 0.75 +/- 0.16 U/mL, whereas the I-inhibin concentration was 9.58 +/- 0.75 U/mL, which was much higher than that of B-inhibin (P less than 0.001). No difference was found in B-inhibin or I-inhibin levels in the serum samples collected separately from umbilical arteries and veins (P greater than 0.05). In addition, inhibin levels were measured in amniotic fluid and placental extract. In amniotic fluid, B-inhibin and I-inhibin concentrations were 2.37 +/- 0.35 and 8.01 +/- 0.55 U/mL, respectively. In the comparison, B-inhibin in amniotic fluid was 31.3% lower than that in maternal serum before delivery (P less than 0.05), but I-inhibin was 112.5% higher than that in the maternal serum (P less than 0.001). In the placental extract, the B-inhibin concentration was 12.80 +/- 0.35 U/g tissue, while the I-inhibin concentration was 33.86 +/- 2.93 U/g tissue, which was more than twice as high as the B-inhibin level (P less than 0.001). Our data provide further evidence that inhibin may be mainly produced in the placenta during pregnancy.
采用绵羊垂体细胞培养和免疫酶分析系统，研究了健康足月孕妇分娩前及分娩后24小时血清中生物抑制素（B - 抑制素）和免疫抑制素（I - 抑制素）水平的变化。在母血中，分娩后最初6小时内，B - 抑制素和I - 抑制素水平均急剧下降。分娩前母血中B - 抑制素和I - 抑制素分别为3.45±0.25和3.77±0.43 U/mL，分娩后30分钟分别下降了35.82%和38.89%。分娩后1小时，B - 抑制素和I - 抑制素分别降低了56.74%和61.48%。6小时后，B - 抑制素和I - 抑制素水平分别降低了93.86%和78.90%。24小时后，两种抑制素几乎检测不到。在胎盘后血清中，B - 抑制素和I - 抑制素分别为4.92±0.34和10.13±1.16 U/mL，即分别比分娩前母血中B - 抑制素水平高42.48%（P＜0.05），比I - 抑制素水平高168.60%（P＜0.001）。在脐血中，B - 抑制素浓度为0.75±0.16 U/mL，而I - 抑制素浓度为9.58±0.75 U/mL，远高于B - 抑制素（P＜0.001）。从脐动脉和脐静脉分别采集的血清样本中，B - 抑制素或I - 抑制素水平无差异（P＞0.05）。此外，还检测了羊水和胎盘提取物中的抑制素水平。羊水中B - 抑制素和I - 抑制素浓度分别为2.37±0.35和8.01±0.55 U/mL。相比之下，羊水中的B - 抑制素比分娩前母血中的低31.3%（P＜0.05），但I - 抑制素比母血中的高112.5%（P＜0.001）。胎盘提取物中，B - 抑制素浓度为12.80±0.35 U/g组织，而I - 抑制素浓度为33.86±2.93 U/g组织，是B - 抑制素水平的两倍多（P＜0.001）。我们的数据进一步证明，孕期抑制素可能主要由胎盘产生。
