Wallace E M, Riley S C, Crossley J A, Ritoe S C, Horne A, Shade M, Ellis P M, Aitken D A, Groome N P
Department of Obstetrics and Gynecology, University of Edinburgh, United Kingdom.
J Clin Endocrinol Metab. 1997 Jan;82(1):218-22. doi: 10.1210/jcem.82.1.3685.
Using new specific and sensitive enzyme-linked immunosorbent assays for inhibin A and inhibin B, we measured these proteins in amniotic fluid (AF), maternal serum (MS), and umbilical cord serum in normal pregnancies. Inhibin A levels in AF rose from a median (10-90th percentile) level of 615 (158.2-1124.6) pg/mL at 14 weeks to 1336.0 (489.4-2084.1) pg/mL at 20 weeks, and inhibin B rose from 216.6 (67.4-554.6) to 1078.2 (439.3-2482.2) pg/mL over the same period. In MS, inhibin A levels fell from a median (10-90th percentile) level of 177.5 (101.4-290.7) pg/mL at 10 weeks to a nadir of 111.9 (59.5-200.3) pg/mL at 17 weeks, rising again to 180.3 (74.1-327.2) pg/mL at 20 weeks. No inhibin B was detectable in MS. In 47 pairs of matched samples (14-16 weeks gestation) there was no correlation of inhibin A levels in AF with those in MS (r = 0.19; P > 0.05). In 45 term umbilical cord serum samples, no dimeric inhibin was detectable in serum from female babies, but inhibin B was detectable in male sera; the median (10-90th percentile) concentration was 167.4 (111.2-224.8) pg/mL. These data suggest that for the gestation periods studied, although the placenta secretes inhibin A, another source, probably the fetal membranes, secretes both inhibin A and inhibin B. Further, the presence of inhibin B in male fetuses is consistent with a testicular origin, suggesting that inhibin B may be important in the development of the fetal hypothalamo-pituitary-testicular axis.
我们使用针对抑制素A和抑制素B的新型特异性和敏感性酶联免疫吸附测定法，在正常妊娠的羊水（AF）、母血（MS）和脐血中测量了这些蛋白质。AF中抑制素A水平从14周时的中位数（第10 - 90百分位数）615（158.2 - 1124.6）pg/mL升至20周时的1336.0（489.4 - 2084.1）pg/mL，抑制素B在同一时期从216.6（67.4 - 554.6）升至1078.2（439.3 - 2482.2）pg/mL。在MS中，抑制素A水平从10周时的中位数（第10 - 90百分位数）177.5（101.4 - 290.7）pg/mL降至17周时的最低点111.9（59.5 - 200.3）pg/mL，然后在20周时再次升至180.3（74.1 - 327.2）pg/mL。在MS中未检测到抑制素B。在47对匹配样本（妊娠14 - 16周）中，AF中的抑制素A水平与MS中的抑制素A水平无相关性（r = 0.19；P > 0.05）。在45份足月脐血样本中，女婴血清中未检测到二聚体抑制素，但男婴血清中可检测到抑制素B；中位数（第10 - 90百分位数）浓度为167.4（111.2 - 224.8）pg/mL。这些数据表明，在所研究的妊娠期，尽管胎盘分泌抑制素A，但另一个来源，可能是胎膜，同时分泌抑制素A和抑制素B。此外，男胎中存在抑制素B与睾丸来源一致，表明抑制素B可能在胎儿下丘脑 - 垂体 - 睾丸轴的发育中起重要作用。
