Barralet J E, Wang L, Lawson M, Triffitt J T, Cooper P R, Shelton R M
Faculty of Dentistry, McGill University, Strathcona Anatomy & Dentistry Building, 3640 University Street, Montreal, Quebec, H3A 2B2, Canada.
J Mater Sci Mater Med. 2005 Jun;16(6):515-9. doi: 10.1007/s10856-005-0526-z.
Calcium cross-linked sodium alginate hydrogels have several advantageous features making them potentially suitable as tissue engineering scaffolds and this material has been previously used in many biomedical applications. 3D cell culture systems are often very different from 2D petri dish type cultures. in this study the effect of alginate hydrogel architecture was investigated by comparing rat bone marrow cell proliferation and differentiation on calcium cross linked sodium alginate discs and 1mm internal diameter tubes. It was found that bone marrow cell proliferation was diminished as the concentration of alginate in the 2D hydrogel substrates increased, yet proliferation was extensive on tubular alginate constructs with high alginate contents. Alginate gel thickness was found to be an important parameter in determining cell behaviour and the different geometries did not generate significant alterations in BMC differentiation profiles.
钙交联海藻酸钠水凝胶具有几个有利特性，使其有可能适合作为组织工程支架，并且这种材料此前已用于许多生物医学应用中。三维细胞培养系统通常与二维培养皿类型的培养有很大不同。在本研究中，通过比较大鼠骨髓细胞在钙交联海藻酸钠圆盘和内径1mm的管子上的增殖和分化，研究了海藻酸钠水凝胶结构的影响。结果发现，随着二维水凝胶基质中海藻酸钠浓度的增加，骨髓细胞增殖减少，但在海藻酸钠含量高的管状海藻酸盐构建体上增殖广泛。发现海藻酸盐凝胶厚度是决定细胞行为的一个重要参数，并且不同的几何形状在骨髓细胞分化谱中没有产生显著变化。
