某些肿瘤相关因子在人类致癌作用及胃癌发生发展中的表达

Expression of some tumor associated factors in human carcinogenesis and development of gastric carcinoma.

作者信息

Zhao Ming-Dong, Hu Xue-Mei, Sun Dian-Jing, Zhang Qun, Zhang Yu-Hao, Meng Wei

机构信息

Department of Radiology, Affiliated Hospital of Binzhou Medical College, Binzhou 256-603, Shandong Province, China.

出版信息

World J Gastroenterol. 2005 Jun 7;11(21):3217-21. doi: 10.3748/wjg.v11.i21.3217.

DOI:10.3748/wjg.v11.i21.3217

PMID:15929170

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4316051/

Abstract

AIM

To study the effect of IGF-1/IGF-1R and gastrin/CCK-BR on carcinogenesis and development of human gastric carcinoma and to explore its mechanism and provide a credible theoretical foundation for early diagnosis and molecular therapy of gastric carcinoma.

METHODS

mRNA expression levels of IGF-1/IGF-1R and gastrin/CCK-BR were assessed by RT-PCR method in gastric cancer tissues, adjacent mucosa, and tumor-free tissues from 56 patients with gastric carcinoma and normal gastric mucosae from 56 healthy controls. Tissue specimens were obtained by biopsy and confirmed by histological evaluation.

RESULTS

The mRNA levels of IGF-1/IGF-1R were increased in gastric cancer tissues compared with normal tissues from healthy controls and successively increased in tumor-free tissues, adjacent mucosa, and gastric cancer tissues. The mRNA levels of gastrin/CCK-BR were increased in gastric cancer tissues compared with normal tissues from healthy controls. There was a significant difference between gastric cancer tissues and adjacent mucosa and tumor-free tissues, but the mRNA levels of gastrin were not significantly increased in adjacent mucosa and gastric cancer tissues compared with tumor-free tissues. The mRNA levels of CCK-BR were increased in gastric cancer tissues and adjacent mucosa compared with tumor-free tissues, but not significantly increased in adjacent mucosa and gastric cancer tissues compared with gastric cancer tissues.

CONCLUSION

Overexpression of IGF-1/IGF-1R and gastrin/CCK-BR promotes the disorderly proliferation of gastric mucosa epithelia and it is of great significance in the carcinogenesis and development of gastric carcinoma.

摘要

目的

研究胰岛素样生长因子-1/胰岛素样生长因子-1受体（IGF-1/IGF-1R）和胃泌素/胆囊收缩素B受体（gastrin/CCK-BR）对人胃癌发生发展的影响，探讨其作用机制，为胃癌的早期诊断和分子治疗提供可靠的理论依据。

方法

采用逆转录聚合酶链反应（RT-PCR）法检测56例胃癌患者的癌组织、癌旁黏膜及癌周无瘤组织以及56例健康对照者正常胃黏膜中IGF-1/IGF-1R和gastrin/CCK-BR的mRNA表达水平。组织标本通过活检获取，并经组织学评估证实。

结果

与健康对照者的正常组织相比，胃癌组织中IGF-1/IGF-1R的mRNA水平升高，且在癌周无瘤组织、癌旁黏膜及癌组织中依次升高。与健康对照者的正常组织相比，胃癌组织中gastrin/CCK-BR的mRNA水平升高。胃癌组织与癌旁黏膜及癌周无瘤组织之间存在显著差异，但与癌周无瘤组织相比，癌旁黏膜和癌组织中胃泌素的mRNA水平无显著升高。与癌周无瘤组织相比，胃癌组织和癌旁黏膜中CCK-BR的mRNA水平升高，但与癌组织相比，癌旁黏膜和癌组织中CCK-BR的mRNA水平升高不显著。