胃癌中胆囊收缩素、胃泌素、CCK - A受体及CCK - B/胃泌素受体基因表达的评估

Evaluation of cholecystokinin, gastrin, CCK-A receptor, and CCK-B/gastrin receptor gene expressions in gastric cancer.

作者信息

Okada N, Kubota A, Imamura T, Suwa H, Kawaguchi Y, Ohshio G, Seino Y, Imamura M

机构信息

First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.

出版信息

Cancer Lett. 1996 Sep 10;106(2):257-62. doi: 10.1016/0304-3835(96)04325-x.

DOI:10.1016/0304-3835(96)04325-x

PMID:8844981

Abstract

The brain-gut hormones, cholecystokinin (CCK) and gastrin, regulate the growth of gastrointestinal mucosa and tumor cells. In this study, reverse transcription-polymerase chain reaction (RT-PCR) was used to evaluate messenger RNA expression for CCK, gastrin, CCK-A receptor, and CCK-B/gastrin receptor in surgical specimens of gastric cancers and in normal antrum and body mucosa of the stomach. The CCK mRNA expression was detectable in 4/14 (29%) samples of gastric cancer and in 3/12 (25%) samples of antral mucosa. However, the gastrin mRNA expression was not detectable in any gastric cancer samples, although it was detectable in all the samples of antral mucosa. The CCK-A receptor mRNA expression was detectable in 5/14 (36%) samples of gastric cancer and in 7/12 (58%) body mucosa. Three cases out of 14 (21%) of gastric cancer expressed both CCK gene and CCK-A receptor gene. The CCK-B receptor mRNA expression was detectable in only 1/14 (7%) samples of gastric cancer, although it was detectable in 10/12 (83%) body mucosa of the stomach. These findings may suggest a greater role for CCK and CCK-A receptor than for gastrin and CCK-B receptor in gastric cancers.

摘要

脑肠肽胆囊收缩素（CCK）和胃泌素可调节胃肠道黏膜及肿瘤细胞的生长。在本研究中，采用逆转录-聚合酶链反应（RT-PCR）来评估胃癌手术标本以及胃正常胃窦和胃体黏膜中CCK、胃泌素、CCK-A受体和CCK-B/胃泌素受体的信使核糖核酸（mRNA）表达。CCK mRNA表达在14份胃癌样本中的4份（29%）以及12份胃窦黏膜样本中的3份（25%）中可检测到。然而，胃泌素mRNA表达在任何胃癌样本中均未检测到，尽管在所有胃窦黏膜样本中均可检测到。CCK-A受体mRNA表达在14份胃癌样本中的5份（36%）以及12份胃体黏膜样本中的7份（58%）中可检测到。14例胃癌中有3例（21%）同时表达CCK基因和CCK-A受体基因。CCK-B受体mRNA表达仅在14份胃癌样本中的1份（7%）中可检测到，尽管在12份胃体黏膜样本中的10份（83%）中可检测到。这些发现可能提示在胃癌中CCK和CCK-A受体比胃泌素和CCK-B受体发挥着更大的作用。