苜蓿根瘤菌共生启动子的缺失分析
Deletion analysis of Rhizobium meliloti symbiotic promoters.
作者信息
Better M, Ditta G, Helinski D R
机构信息
Department of Biology, B-022, University of California, San Diego, La Jolla, CA 92093, USA.
出版信息
EMBO J. 1985 Oct;4(10):2419-24. doi: 10.1002/j.1460-2075.1985.tb03950.x.
Abstract
Previous examination of DNA sequences located 5' to Rhizobium meliloti nif transcription units has shown that extensive sequence homology exists among them. Here we have examined these reiterated sequences for their role in symbiotic gene regulation. Promoter deletion analysis has shown that although an extensive upstream DNA sequence 9160 bp) is required for full heterologous activation of the R. meliloti nifHDK promoter by the Klebsiella pneumoniae nifA protein in Escherichia coli, this region is not required for expression of R. Meliloti nif promoters in root nodules from plants grown under greenhouse conditions. In addition, a minimum functional symbiotic promoter sequence is defined, and a DNA sequence difference affecting regulation of two symbiotic promoters is discussed.
摘要
先前对位于苜蓿根瘤菌固氮转录单元5'端的DNA序列进行的研究表明，它们之间存在广泛的序列同源性。在此，我们研究了这些重复序列在共生基因调控中的作用。启动子缺失分析表明，尽管肺炎克雷伯菌的nifA蛋白在大肠杆菌中对苜蓿根瘤菌nifHDK启动子进行完全异源激活需要一段9160 bp的广泛上游DNA序列，但在温室条件下生长的植物根瘤中，该区域对于苜蓿根瘤菌nif启动子的表达并非必需。此外，还定义了一个最小功能共生启动子序列，并讨论了影响两个共生启动子调控的DNA序列差异。
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本文引用的文献
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