葛根（野葛）中的一种糖苷类黄酮：鉴定、定量及抗氧化活性测定

A glycoside flavonoid in Kudzu (Pueraria lobata): identification, quantification, and determination of antioxidant activity.

作者信息

Lau Ching S, Carrier Danielle J, Beitle Robert R, Howard Luke R, Lay Jackson O, Liyanage Rohana, Clausen Edgar C

机构信息

Department of Chemical Engineering, 3202 Bell Engineering Center, University of Arkansas, 115 Chemistry Building, Fayetteville, AR 72701, USA.

出版信息

Appl Biochem Biotechnol. 2005 Spring;121-124:783-94. doi: 10.1385/abab:123:1-3:0783.

DOI:10.1385/abab:123:1-3:0783

PMID:15930558

Abstract

Kudzu (Pueraria lobata) foliage has been touted as a possible energy crop. High-performance liquid chromatography and mass spectrometry analysis of the methanolic kudzu foliage extracts confirmed the presence of robinin (kaempferol-3-O-robinoside-7-O-rhamnoside). Robinin accounted for 0.65 +/- 0.16% (dry basis) of kudzu biomass. Fast performance liquid chromatography (FPLC) was employed to fractionate robinin from the crude extract. The antioxidant capacity of robinin was evaluated by an oxygen radical absorbance capacity (ORAC) assay. The ORAC values of pure standard were compared with those of the extract fractions. One milligram of the FPLC-fractionated robinin generated an ORAC value of 5.15 +/- 2.00 micromol/mg of Trolox, whereas 1 mg of pure robinin generated an ORAC value of 12.34 +/- 0.45 micromol/mg of Trolox. Because of its antioxidant properties, robinin may be a flavonoid worth extracting prior to energy production.

摘要

葛根（野葛）的叶子被认为是一种潜在的能源作物。对葛根叶甲醇提取物进行高效液相色谱和质谱分析，证实了其中存在刺槐苷（山奈酚-3-O-刺槐糖苷-7-O-鼠李糖苷）。刺槐苷占葛根生物质的0.65±0.16%（干基）。采用快速液相色谱（FPLC）从粗提物中分离刺槐苷。通过氧自由基吸收能力（ORAC）测定法评估刺槐苷的抗氧化能力。将纯标准品的ORAC值与提取物馏分的ORAC值进行比较。1毫克经FPLC分离的刺槐苷产生的ORAC值为5.15±2.00微摩尔/毫克Trolox，而1毫克纯刺槐苷产生的ORAC值为12.34±0.45微摩尔/毫克Trolox。由于其抗氧化特性，刺槐苷可能是一种在能源生产之前值得提取的黄酮类化合物。