Liang Qingrong, Qian He, Yao Weirong
Department of Food Science and Technology,Southern Yangtze University, Wuxi 214036, China.
Eur J Mass Spectrom (Chichester). 2005;11(1):93-101. doi: 10.1255/ejms.710.
Identification of flavonoids and flavonoid glycosides was carried out on Psidium guajava Linn leaves by means of high-performance liquid chromatography ultraviolet (HPLC-UV) analysis and HPLC mass spectrometry. By using HPLC-UV, two known phenolics (gallic acid and quercetin) and five newly reported ones (procatechuic acid, chlorogenic acid, caffeic acid, kaempferol and ferulic acid) were identified in alcohol guava leaf extract. Structural information about the compounds was obtained from the retention times, the UV spectra and mass spectra without the need to isolate the individual compounds. Two flavonoids (quercetin and kaempferol) and four flavonoid glycosides (three known components, quercetin 3-O-alpha-L-arabinoside, quercetin 3-O-beta-D-glucoside and quercetin 3-O-beta-D-galactoside, along with one novel compound, kaempferol-glycoside) and three other unknown compounds have been identified in the fractions.
通过高效液相色谱-紫外（HPLC-UV）分析和HPLC质谱法对番石榴叶中的黄酮类化合物和黄酮苷进行了鉴定。利用HPLC-UV，在番石榴叶乙醇提取物中鉴定出两种已知酚类物质（没食子酸和槲皮素）以及五种新报道的酚类物质（原儿茶酸、绿原酸、咖啡酸、山奈酚和阿魏酸）。无需分离单个化合物，即可从保留时间、紫外光谱和质谱中获得这些化合物的结构信息。在这些馏分中已鉴定出两种黄酮类化合物（槲皮素和山奈酚）、四种黄酮苷（三种已知成分，槲皮素3-O-α-L-阿拉伯糖苷、槲皮素3-O-β-D-葡萄糖苷和槲皮素3-O-β-D-半乳糖苷，以及一种新化合物，山奈酚-糖苷）和其他三种未知化合物。
