Rotariu Ovidiu, Ogden Iain D, MacRae Marion, Udrea Laura Elena, Strachan Norval J C
School of Biological Sciences, University of Aberdeen, Cruickshank Building, St. Machar Drive, Aberdeen, UK.
Phys Med Biol. 2005 Jun 21;50(12):2967-77. doi: 10.1088/0031-9155/50/12/016. Epub 2005 Jun 1.
The standard method of immunomagnetic separation for isolating pathogenic bacteria from food and environmental matrices processes 1 ml volumes. Pathogens present at low levels (<0.5 pathogenic bacteria/g) will not be consistently detected by this method. Here a multiple sample flow through immunomagnetic separator has been designed and tested to process large volume samples (50 to 250 ml). Preliminary results show >97% recovery of polydisperse magnetic particles (diameter range 1 to 8 microm) containing 29-33% w/w Fe3O4 content. Between 70 and 130 times more of the pathogenic bacteria Escherichia coli O157 is recovered from PBS compared with the standard 1 ml method. Also, the recovery of E. coli O157 from beef mince homogenates, after a 4 h incubation at 42 degrees C, is between 80 and 180 times higher than the standard 1 ml method.
从食品和环境基质中分离致病细菌的免疫磁分离标准方法处理的样本体积为1毫升。该方法无法始终检测到低水平存在的病原体（<0.5个致病细菌/克）。在此，设计并测试了一种多样本流通式免疫磁分离器，用于处理大体积样本（50至250毫升）。初步结果表明，含有29-33% w/w Fe3O4的多分散磁性颗粒（直径范围为1至8微米）的回收率>97%。与标准的1毫升方法相比，从磷酸盐缓冲盐水中回收的致病细菌大肠杆菌O157多70至130倍。此外，在42摄氏度下孵育4小时后，从牛肉末匀浆中回收的大肠杆菌O157比标准的1毫升方法高80至180倍。
