Helliwell John R
Department of Chemistry, University of Manchester, M13 9PL, England.
Acta Crystallogr D Biol Crystallogr. 2005 Jun;61(Pt 6):793-8. doi: 10.1107/S0907444905001368. Epub 2005 May 26.
Knowledge of protein crystal perfection in theory and practice is reviewed. X-ray methods of assessing perfection such as mosaicity, topography and reciprocal-space mapping are described. X-ray diffraction physics applications of protein crystals such as in Laue geometry, the large-angle oscillation technique and forming polychromatic profiles across diffraction spots are covered. Cryo- and room-temperature cases are discussed including in X-ray and neutron protein crystallography. Experience of freezing very large crystals, which are commonly used today in neutron protein crystallography, is highlighted.
本文综述了蛋白质晶体完整性在理论和实践方面的知识。描述了评估晶体完整性的X射线方法，如镶嵌性、形貌和倒易空间映射。涵盖了蛋白质晶体在劳厄几何、大角度振荡技术以及在衍射斑点上形成多色轮廓等方面的X射线衍射物理应用。讨论了低温和室温条件下的情况，包括X射线和中子蛋白质晶体学。重点介绍了如今在中子蛋白质晶体学中常用的冷冻非常大的晶体的经验。
