培养基成分对破伤风梭菌产生破伤风毒素的影响。

Effect of medium composition on the production of tetanus toxin by Clostridium tetani.

作者信息

Fratelli Fernando, Siquini Tatiana Joly, Prado Sally Müller Affonso, Higashi Hisako Gondo, Converti Attilio, de Carvalho João Carlos Monteiro

机构信息

Division of Technology and Production Development of Institute Butantan, Av. Vital Brazil, 1500, 05504-900 São Paulo-SP, Brazil.

出版信息

Biotechnol Prog. 2005 May-Jun;21(3):756-61. doi: 10.1021/bp049571b.

DOI:10.1021/bp049571b

PMID:15932253

Abstract

The tetanus toxin is a neurotoxin synthesized by the bacillus Clostridium tetani that, after detoxification with formaldehyde, still exhibits antigenic and immunologic properties, hence its denomination of tetanus toxoid. Such a neurotoxin is produced by cultivation of the microorganism in vegetative form on a relatively complex specific medium containing glucose and peptone. The simultaneous effects of the starting levels of glucose (G0) and N-Z Case TT (NZ0) as carbon and nitrogen sources, respectively, on the production of tetanus toxin have been investigated in this work in static cultivations by means of a five-level star-shaped experimental design and evaluated by response surface methodology (RSM) for optimization purposes. The highest final average yield of tetanus toxin (72 Lf/mL), achieved at G0= 9.7 g/L and NZ0= 43.5 g/L, was 80% higher than that obtained with standard cultivations (G0= 8.0 g/L and NZ0= 25.0 g/L).

摘要

破伤风毒素是由破伤风梭菌合成的一种神经毒素，在用甲醛解毒后，它仍具有抗原性和免疫特性，因此被命名为破伤风类毒素。这种神经毒素是通过在含有葡萄糖和蛋白胨的相对复杂的特定培养基上以营养形式培养微生物产生的。在这项工作中，通过五级星形实验设计，在静态培养中研究了分别作为碳源和氮源的葡萄糖起始水平（G0）和N-Z酪蛋白胰消化物（NZ0）对破伤风毒素产生的同时影响，并通过响应面法（RSM）进行评估以达到优化目的。在G0 = 9.7 g/L和NZ0 = 43.5 g/L时获得的破伤风毒素最高最终平均产量（72 Lf/mL）比标准培养（G0 = 8.0 g/L和NZ0 = 25.0 g/L）所获得的产量高80%。