在破伤风梭菌培养过程中通入氮气气泡可提高破伤风毒素的产量，用于制备类毒素。

Nitrogen-gas bubbling during the cultivation of Clostridium tetani produces a higher yield of tetanus toxin for the preparation of its toxoid.

作者信息

De Luca M M, Abeiro H D, Bernagozzi J A, Basualdo J A

机构信息

Cátedra de Microbiología y Parasitología, Facultad de Ciencias Médicas, Universidad Nacional de la Plata (UNLP), República Argentina.

出版信息

Microbiol Immunol. 1997;41(2):161-3. doi: 10.1111/j.1348-0421.1997.tb01181.x.

DOI:10.1111/j.1348-0421.1997.tb01181.x

PMID:9087958

Abstract

We investigated the effect of exposing cultures of Clostridium tetani to nitrogen (N2) gas on the recovery of tetanus toxin to be processed for the preparation of its toxoid. N2 was bubbled through nine 10-liter cultures during the growth of the bacteria, while nine parallel control incubations were maintained without bubbling. We found that treatment of the C. tetani anaerobes with an inert gas in this manner during cultivation produced a highly significant increase in the yield of tetanus toxin from them in comparison with the standard procedure.

摘要

我们研究了将破伤风梭菌培养物暴露于氮气（N₂）气体对用于制备类毒素的破伤风毒素回收率的影响。在细菌生长期间，将氮气通入9个10升的培养物中，同时维持9个平行的对照培养物不进行通气。我们发现，与标准程序相比，在培养过程中以这种方式用惰性气体处理破伤风梭菌厌氧菌可使其破伤风毒素产量显著提高。