Lee In Su, Bae Hee-Sung, Ryu Hee Wook, Cho Kyung-Suk, Chang Yong Keun
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea.
Biotechnol Prog. 2005 May-Jun;21(3):781-5. doi: 10.1021/bp0496171.
A new type of air-lift reactor with immobilized Gordonia nitida CYKS1 cells on a fibrous support was designed and used for the biocatalytic desulfurization (BDS) of diesel oil. Its performance was evaluated at different phase ratios of the oil to the aqueous medium (or oil phase fractions) and different sucrose concentrations. When the reaction mixture contained 10% diesel oil (v/v), 61-67% of sulfur was removed as the sulfur content decreased from 202-250 to 76-90 mg L(-1) in 72 h. The sulfur content did not decrease any further because the remaining sulfur compounds were recalcitrant to BDS. During the desulfurization, the strain CYKS1 consumed hydrocarbons in the diesel oil, mainly n-alkanes with 10-26 carbons, as carbon source even though an easily available carbon source, sucrose, was supplied.
设计了一种新型气升式反应器，该反应器将光亮戈登氏菌CYKS1细胞固定在纤维载体上，并用于柴油的生物催化脱硫（BDS）。在油与水相介质的不同相比（或油相分数）以及不同蔗糖浓度下对其性能进行了评估。当反应混合物含有10%（v/v）的柴油时，在72小时内，随着硫含量从202 - 250毫克/升降至76 - 90毫克/升，61 - 67%的硫被去除。硫含量没有进一步降低，因为剩余的硫化合物对生物催化脱硫具有抗性。在脱硫过程中，尽管提供了易于利用的碳源蔗糖，但菌株CYKS1消耗柴油中的碳氢化合物作为碳源，主要是含10 - 26个碳的正构烷烃。
