School of Chemical Engineering, Department of Life Science Engineering, University College of Engineering, University of Tehran, P.O. Box 11155-4563, Tehran, Iran.
Bioresour Technol. 2011 Nov;102(22):10585-91. doi: 10.1016/j.biortech.2011.08.120. Epub 2011 Sep 5.
For the first time, growing cells of Gordonia alkanivorans RIPI90A were used for biodesulfurization (BDS) of diesel. This process was carried out in an internal airlift bioreactor. BDS parameters (oil/water phase ratio and initial sulfur concentration) were optimized in flasks using response surface methodology. Predicted results were found to be in good agreement with experimental results. Initial sulfur concentration had a remarkable effect on BDS process. Maximum removal of sulfur (21 mg/l) can be achieved at oil/water phase ratio of 25% (v/v) and initial sulfur concentration of 28 mg/l. Moreover, effect of superficial gas velocity (Ug) and working volume (v) on volumetric gas liquid mass transfer coefficient was studied in an airlift bioreactor for BDS of diesel. The best results were achieved at Ug and v of 2.5l/min and 6.6l, respectively. Subsequently, BDS of diesel was investigated in an airlift bioreactor under optimized conditions. Sulfur reduction after 30 h was 14 mg/l.
首次利用戈登氏菌（ Gordonia alkanivorans ） RIPI90A 细胞进行生物脱硫（ BDS ）实验，以柴油为处理对象。该过程在内部气升式生物反应器中进行。采用响应面法在摇瓶中对 BDS 参数（油/水相比例和初始硫浓度）进行了优化。预测结果与实验结果吻合较好。初始硫浓度对 BDS 过程有显著影响。在油/水相比例为 25%（v/v ）和初始硫浓度为 28mg/L 时，可达到最大脱硫率（ 21mg/L ）。此外，还研究了气升式生物反应器中表观气速（ Ug ）和工作体积（ v ）对柴油生物脱硫体积气液传质系数的影响。在 Ug 和 v 分别为 2.5l/min 和 6.6l 时，获得了最佳结果。随后，在优化条件下，在气升式生物反应器中进行了柴油的 BDS 实验。 30h 后，硫的还原量为 14mg/L 。
