Noonan James P, Hofreiter Michael, Smith Doug, Priest James R, Rohland Nadin, Rabeder Gernot, Krause Johannes, Detter J Chris, Pääbo Svante, Rubin Edward M
United States Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA.
Science. 2005 Jul 22;309(5734):597-9. doi: 10.1126/science.1113485. Epub 2005 Jun 2.
Despite the greater information content of genomic DNA, ancient DNA studies have largely been limited to the amplification of mitochondrial sequences. Here we describe metagenomic libraries constructed with unamplified DNA extracted from skeletal remains of two 40,000-year-old extinct cave bears. Analysis of approximately 1 megabase of sequence from each library showed that despite significant microbial contamination, 5.8 and 1.1% of clones contained cave bear inserts, yielding 26,861 base pairs of cave bear genome sequence. Comparison of cave bear and modern bear sequences revealed the evolutionary relationship of these lineages. The metagenomic approach used here establishes the feasibility of ancient DNA genome sequencing programs.
尽管基因组DNA含有更多信息，但古代DNA研究在很大程度上局限于线粒体序列的扩增。在此，我们描述了用从未经扩增的DNA构建的宏基因组文库，这些DNA取自两只生活在4万年前现已灭绝的洞熊的骨骼遗骸。对每个文库中约1兆碱基的序列分析表明，尽管存在大量微生物污染，但5.8%和1.1%的克隆含有洞熊插入片段，从而获得了26,861个碱基对的洞熊基因组序列。洞熊与现代熊序列的比较揭示了这些谱系的进化关系。这里使用的宏基因组方法确立了古代DNA基因组测序计划的可行性。
