脐带血CD34+和CD90+干细胞与新生儿因素的关联：一项横断面研究
Association of CD34+ and CD90+ Stem Cells of Cord Blood with Neonatal Factors: A Cross-sectional Study.
作者信息
Mantri Santwana, Rao E Venkata, Jena Pradeep Kumar, Mohapatra Prakash Chandra
机构信息
Department of Biochemistry, S.C.B. Medical College, Mangalabag, Cuttack, Odisha, 751003, India.
Department of Community Medicine, Institute of Medical Sciences & SUM Hospital, Bhubaneswar, Orissa, India.
出版信息
Indian J Pediatr. 2016 Feb;83(2):114-9. doi: 10.1007/s12098-015-1839-7. Epub 2015 Aug 7.
OBJECTIVE
To characterize the primitive stem cell content of cord blood with regard to neonatal parameters.
METHODS
In this cross-sectional study, CD34+ and CD90+ cells content were enumerated by flow-cytometry method. Their associations with various neonatal parameters like birth weight, gender, gestational age and mode of delivery were analyzed by univariate analysis. Multivariable linear regression model was then developed to further explain the effect of neonatal factors on these primitive cell counts.
RESULTS
From a total of 106 recruited subjects, gender of the neonate did not have any influence on the expression of these proteins (CD34 and CD90) of cord blood stem cells or progenitors. Multi variable linear regression analysis using CD34+ and CD90+ cell counts as dependent variables revealed that birth weight and the mode of delivery were significant predictors of these cell counts.
CONCLUSIONS
The present study suggests that birth weight and mode of delivery of the neonates influences cord blood stem cell yield.
目的
根据新生儿参数描述脐带血原始干细胞的含量。
方法
在这项横断面研究中，采用流式细胞术方法对CD34+和CD90+细胞含量进行计数。通过单因素分析分析它们与各种新生儿参数（如出生体重、性别、胎龄和分娩方式）的关联。然后建立多变量线性回归模型，以进一步解释新生儿因素对这些原始细胞计数的影响。
结果
在总共106名招募的受试者中，新生儿的性别对脐带血干细胞或祖细胞这些蛋白质（CD34和CD90）的表达没有任何影响。以CD34+和CD90+细胞计数作为因变量的多变量线性回归分析显示，出生体重和分娩方式是这些细胞计数的重要预测因素。
结论
本研究表明，新生儿的出生体重和分娩方式会影响脐带血干细胞的产量。
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