Bakst M R, Cecil H C
US Department of Agriculture, Agricultural Research Service, Avian Physiology Laboratory, Beltsville, MD 20705.
J Reprod Fertil. 1992 Mar;94(2):287-93. doi: 10.1530/jrf.0.0940287.
Motility characteristics of turkey spermatozoa before and after storage for 24 h at 7 degrees C in diluent with and without bovine serum albumin (BSA; 1% final concentration) were measured by computer-assisted semen analysis. BSA significantly increased the percentage of motile spermatozoa and sperm velocity, linearity, lateral head displacement and beat frequency in each treatment, but BSA in fresh or stored semen in diluent did not augment hen fertility over 15 weeks of egg production. Fatty-acid-free BSA, globulin-free BSA and Fraction V BSA all significantly increased each sperm motility characteristic compared with semen in diluent alone. The lack of correlation between sperm motility and fecundity emphasizes the need to develop procedures for semen evaluation that accurately predict the fertilizing capacity of an aliquot of semen.
通过计算机辅助精液分析，测定了火鸡精子在含有和不含牛血清白蛋白（BSA；终浓度1%）的稀释液中于7℃储存24小时前后的活力特征。在每种处理中，BSA显著提高了活动精子的百分比以及精子速度、直线性、头部侧向位移和鞭打频率，但在产蛋15周期间，稀释液中新鲜或储存精液中的BSA并未提高母鸡的受精率。与仅在稀释液中的精液相比，无脂肪酸BSA、无球蛋白BSA和V组分BSA均显著提高了每种精子活力特征。精子活力与繁殖力之间缺乏相关性，这强调了开发准确预测一份精液受精能力的精液评估程序的必要性。
