Scorilas Andreas, Agiamarnioti Kalomoira, Papadopoulos Kyriakos
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Athens, 15701 Athens, Greece.
Clin Chim Acta. 2005 Jul 24;357(2):159-67. doi: 10.1016/j.cccn.2005.03.021.
The favorable properties of acridinium derivatives over conventional fluorescent probes have attracted considerable interest. These conjugates have found wide utility in tests developed for medical diagnosis providing at least comparable sensitivity to radioimmunoassay (RIA) and enzyme immunoassay (EIA) procedures.
In this paper we describe the synthesis, luminescent properties and immunoassay applications of two novel biotinylated acridinium derivatives, 9-(2-biotinyl-oxyethyl)-carboxylate-10-methyl-acridinium triflate, BOCMAT and 9-(2-biotinyl-amidoethyl)-carboxylate-10-methyl-acridinium triflate, BACMAT.
The chemiluminescence efficiency of the novel reagents is high in polar aprotic solvents and attains detection limits down to 7.28 x 10(-8) M, while the fluorescence efficiency has its maximum in aqueous solutions with detection limits down to 1.94 x 10(-10) M. The above novel compounds were applied in the fluorimetric determination of solid phase-immobilized biotinylated mouse IgG and the detection limit was found to be approximately 1 microg/assay ( approximately 7 pmol/assay).
The new streptavidin-based detection reagents were synthesized can be used for the development of highly sensitive solid-phase fluorescence-based immunoassays and may find important applications in proteomics.
吖啶鎓衍生物相较于传统荧光探针的优良特性已引起了广泛关注。这些共轭物在医学诊断检测中具有广泛用途，其灵敏度至少与放射免疫分析（RIA）和酶免疫分析（EIA）方法相当。
本文描述了两种新型生物素化吖啶鎓衍生物9-(2-生物素酰氧基乙基)-羧酸盐-10-甲基-吖啶鎓三氟甲磺酸盐（BOCMAT）和9-(2-生物素酰氨基乙基)-羧酸盐-10-甲基-吖啶鎓三氟甲磺酸盐（BACMAT）的合成、发光特性及免疫分析应用。
新型试剂在极性非质子溶剂中的化学发光效率较高，检测限低至7.28×10⁻⁸ M，而荧光效率在水溶液中最高，检测限低至1.94×10⁻¹⁰ M。上述新型化合物应用于荧光法测定固相固定的生物素化小鼠IgG，检测限约为1 μg/测定（约7 pmol/测定）。
合成的新型基于链霉亲和素的检测试剂可用于开发高灵敏度的基于固相荧光的免疫分析，可能在蛋白质组学中找到重要应用。
