Kono T, Kwon O Y, Watanabe T, Nakahara T
NODAI Research Institute, Tokyo, Japan.
J Reprod Fertil. 1992 Mar;94(2):481-7. doi: 10.1530/jrf.0.0940481.
The influence of the stage of the cell cycle of donor nuclei on the development of mouse oocytes enucleated at telophase I was examined. After nuclear transplantation and activation, a high proportion of the oocytes remodelled a nucleus, emitted a polar body and formed a pronuclear-like nucleus. Most of the reconstituted embryos that received an interphase nucleus 30-32 h or 34-36 h after treatment with human chorionic gonadotrophin (hCG) arrested at the 2-cell stage. The reconstituted embryos were able to develop to blastocysts when nuclei from late 2-cell embryos (44-46 and 48-50 h after hCG) were transferred to the oocytes. The resulting blastocysts were transferred to recipients and ten live young were obtained from the embryos that formed a pronuclear-like nucleus after extrusion of a polar body. Thus, the developmental ability of the reconstituted embryos was critically influenced by the stage of the cell cycle of the donor nuclei.
研究了供体细胞核的细胞周期阶段对处于第一次减数分裂末期去核的小鼠卵母细胞发育的影响。核移植和激活后，高比例的卵母细胞重塑了细胞核，排出了极体并形成了类原核细胞核。在用人绒毛膜促性腺激素（hCG）处理后30 - 32小时或34 - 36小时接受间期细胞核的大多数重构胚胎停滞在2 - 细胞阶段。当将来自2 - 细胞晚期胚胎（hCG后44 - 46小时和48 - 50小时）的细胞核转移到卵母细胞时，重构胚胎能够发育到囊胚阶段。将得到的囊胚移植到受体体内，从排出极体后形成类原核细胞核的胚胎中获得了10只活仔。因此，重构胚胎的发育能力受到供体细胞核细胞周期阶段的关键影响。
