Kono T, Kwon O Y, Nakahara T
NODAI Research Institute, Tokyo University of Agriculture, Japan.
J Reprod Fertil. 1991 Sep;93(1):165-72. doi: 10.1530/jrf.0.0930165.
The chromosomes of mouse oocytes at telophase of the first meiotic division were removed using micromanipulation and differential interference microscopy. The enucleated oocytes were used as recipients for nuclear transplantation, after culture for 4-6 h. The newly synthesized proteins of the enucleated oocytes showed the same pattern as those of secondary oocytes matured in vivo. When the enucleated oocytes received a nucleus from late 2- and 8-cell embryos, or a cell from the inner cell mass (ICM) of blastocysts, 23, 4 and 10%, respectively, of reconstituted embryos developed to blastocysts. After transfer to recipient females, live young were produced from the reconstituted eggs that received a nucleus from late 2-cell embryos.
利用显微操作和微分干涉显微镜技术去除处于第一次减数分裂末期的小鼠卵母细胞的染色体。去核卵母细胞在培养4 - 6小时后用作核移植的受体。去核卵母细胞新合成的蛋白质显示出与体内成熟的次级卵母细胞相同的模式。当去核卵母细胞接受来自2 - 8细胞晚期胚胎的细胞核或囊胚内细胞团（ICM）的细胞时，分别有23%、4%和10%的重构胚胎发育到囊胚阶段。将接受来自2 - 8细胞晚期胚胎细胞核的重构卵移植到受体雌性动物体内后，产下了活体幼崽。
