Gómez-Couso H, Amar C F L, McLauchlin J, Ares-Mazás E
Laboratorio de Parasitología, Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, Avenida de Vigo s/n, 15782 Santiago de Compostela, A Coruña, Spain.
Vet Parasitol. 2005 Jul 15;131(1-2):139-44. doi: 10.1016/j.vetpar.2005.04.022.
Cryptosporidium oocysts were detected using a direct immunofluorescence antibody test in the faeces of an asymptomatic water buffalo (Bubalus bubalis) heifer from a dairy farm close to Santiago de Compostela (NW Spain). Oocysts were morphologically indistinguishable from Cryptosporidium parvum. Using DNA extracted from this sample and a PCR-RFLP analysis of a 341 base pairs fragment of the Cryptosporidium oocyst wall protein (COWP) gene, a previously undescribed fragment pattern was generated. The COWP gene fragment was cloned and sequencing analyses revealed it to be similar to the C. parvum 'pig' genotype but with four base pairs substitutions.
在西班牙西北部圣地亚哥-德孔波斯特拉附近一个奶牛场的一头无症状水牛（Bubalus bubalis）小母牛粪便中，采用直接免疫荧光抗体试验检测到隐孢子虫卵囊。这些卵囊在形态上与微小隐孢子虫无法区分。利用从该样本中提取的DNA以及对隐孢子虫卵囊壁蛋白（COWP）基因341个碱基对片段进行的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析，产生了一种以前未描述过的片段模式。对COWP基因片段进行克隆并测序分析后发现，它与微小隐孢子虫“猪”基因型相似，但有四个碱基对替换。
