He Hailun, Chen Xiulan, Sun Caiyun, Zhang Yuzhong, Gao Peiji
State Key Lab of Microbial Technology, Shandong University, Jinan, 250100, China.
Bioresour Technol. 2006 Feb;97(3):385-90. doi: 10.1016/j.biortech.2005.03.016. Epub 2005 Jun 1.
Marine organisms are potentially an untapped source of drugs and value-added food production. Currently, Acetes chinensis is an underutilized shrimp species with low commercial value from the Bo Hai Gulf of China. In this paper, the shrimp was digested by a crude protease from Bacillus sp. SM98011 and filtered through a 3 kDa ultrafiltration membrane. Biological functions of the hydrolysate and ultrafiltrate were then assayed. The analyses showed that 41% of the oligopeptides in the ultrafiltrate had a molecular mass lower than 3 kDa. The antioxidant activities of the hydrolysate and ultrafiltrate were determined through the scavenger activity of the hydroxyl radical, with inhibitions of 42.38% and 67.95%, respectively. The hydrolysate and ultrafiltrate also had good Angiotensin-I-converting enzyme (ACE) inhibitory activity, with IC50 values of 0.98 mg/ml and 0.22 mg/ml, respectively. In addition, Chitin and chitosan were recovered from the hydrolytic sediment using a much smaller volume of strong acids and bases than is normally needed. With this method, we have shown that A. chinensis can be utilized to generate a high value-added product, and have revealed its hidden potential in the production of functional foods and ACE inhibitory peptides.
海洋生物有可能是药物和增值食品生产的未开发来源。目前，中国毛虾是一种未得到充分利用的虾类，在中国渤海湾商业价值较低。本文中，该虾被芽孢杆菌属SM98011的粗蛋白酶消化，并通过3 kDa超滤膜过滤。然后对水解产物和超滤物的生物学功能进行了测定。分析表明，超滤物中41%的寡肽分子量低于3 kDa。通过羟基自由基清除活性测定了水解产物和超滤物的抗氧化活性，抑制率分别为42.38%和67.95%。水解产物和超滤物还具有良好的血管紧张素I转换酶（ACE）抑制活性，IC50值分别为0.98 mg/ml和0.22 mg/ml。此外，从水解沉淀物中回收几丁质和壳聚糖时，所使用的强酸和强碱的体积比通常所需的要小得多。通过这种方法，我们证明了中国毛虾可用于生产高附加值产品，并揭示了其在功能性食品和ACE抑制肽生产中的潜在价值。
