白细胞介素-15对人多形核白细胞抗镰刀菌属和赛多孢子菌属真菌反应的影响

Effects of interleukin-15 on antifungal responses of human polymorphonuclear leukocytes against Fusarium spp. and Scedosporium spp.

作者信息

Winn Richard M, Gil-Lamaignere Cristina, Roilides Emmanuel, Simitsopoulou Maria, Lyman Caron A, Maloukou Avgi, Walsh Thomas J

机构信息

3rd Department of Pediatrics, Aristotle University of Thessaloniki, Hippokration Hospital, GR-54642 Thessaloniki, Greece.

出版信息

Cytokine. 2005 Jul 7;31(1):1-8. doi: 10.1016/j.cyto.2004.07.016.

DOI:10.1016/j.cyto.2004.07.016

PMID:15935692

Abstract

Fusarium spp. and Scedosporium spp. have emerged as important fungal pathogens that are frequently resistant to antifungal compounds. We investigated the effects of human interleukin-15 (IL-15) on human polymorphonuclear leukocyte (PMNL) activity against Fusarium solani and Fusarium oxysporum as well as Scedosporium prolificans and Scedosporium apiospermum. IL-15 (100 ng/ml) significantly enhanced PMNL-induced hyphal damage of both Fusarium spp. and S. prolificans after incubation for 22 h (P < 0.01) but not S. apiospermum. In addition, IL-15 enhanced PMNL oxidative respiratory burst evaluated as superoxide anion production in response to S. prolificans but not to the other fungi after 2 h incubation. IL-15 increased interleukin-8 (IL-8) release from PMNLs challenged with hyphae of F. solani and S. prolificans (P< or = 0.04). Release of tumor necrosis factor-alpha was not affected. The species-dependent enhancement of hyphal damage and induction of IL-8 release suggest that IL-15 plays an important role in the immunomodulation of host response to these emerging fungal pathogens.

摘要

镰刀菌属和多育赛多孢菌已成为重要的真菌病原体，它们常常对抗真菌化合物具有抗性。我们研究了人白细胞介素-15（IL-15）对人多形核白细胞（PMNL）针对茄病镰刀菌、尖孢镰刀菌以及多育赛多孢菌和尖端赛多孢菌活性的影响。孵育22小时后，IL-15（100 ng/ml）显著增强了PMNL诱导的镰刀菌属和多育赛多孢菌的菌丝损伤（P < 0.01），但对尖端赛多孢菌没有影响。此外，孵育2小时后，IL-15增强了PMNL的氧化呼吸爆发，以超氧阴离子产生量来评估，这是针对多育赛多孢菌而言，对其他真菌则没有增强作用。IL-15增加了受茄病镰刀菌和多育赛多孢菌菌丝刺激的PMNLs中白细胞介素-8（IL-8）的释放（P≤0.04）。肿瘤坏死因子-α的释放没有受到影响。菌丝损伤的种属依赖性增强以及IL-8释放的诱导表明，IL-15在宿主对这些新出现的真菌病原体反应的免疫调节中发挥着重要作用。