Gil-Lamaignere Cristina, Winn Richard M, Simitsopoulou Maria, Maloukou Avgi, Walsh Thomas J, Roilides Emmanuel
Third Department of Pediatrics, Aristotle University of Thessaloniki, Hippokration Hospital, Thessaloniki, Greece.
Med Mycol. 2005 May;43(3):253-60. doi: 10.1080/13693780412331271072.
While Aspergillus spp. have been the most frequent filamentous fungi causing infections in immunocompromised patients, Scedosporium spp. are emerging as life-threatening pathogens. We studied the effects of interferon gamma (IFN-gamma) and granulocyte-macrophage colony-stimulating factor (GM-CSF) alone or combined on the antifungal activities of human polymorphonuclear leukocytes (PMN) against Scedosporium apiospermum and Scedosporium prolificans. We paralleled these activities to those against Aspergillus fumigatus and Aspergillus flavus. Incubation of PMN with IFN-gamma and GM-CSF for 22 h enhanced PMN-induced hyphal damage of both Aspergillus spp. and S. prolificans (p < 0.05) but not of S. apiospermum. However, hyphae of S. apiospermum were damaged significantly more after incubation with PMN that had been treated with IFN-gamma and GM-CSF for 2 h. In addition, incubation of PMN with GM-CSF for 2 h enhanced PMN oxidative burst measured as superoxide anion (O2-) production in response to nonopsonized hyphae of A. flavus and Scedosporium spp. (p < 0.05). In contrast, after 2 h, IFN-gamma and GM-CSF alone did not enhance PMN O2- in response to opsonized hyphae of A. flavus and Scedosporium spp.; however, the combination of IFN-gamma and GM-CSF showed significant enhancement against these species. Thus, IFN-gamma and GM-CSF, particularly in combination, demonstrate a species- and time-dependent augmentation of PMN responses to Scedosporium spp.
虽然曲霉属一直是免疫功能低下患者中最常见的引起感染的丝状真菌，但多育赛多孢菌正成为危及生命的病原体。我们研究了单独或联合使用干扰素γ（IFN-γ）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）对人多形核白细胞（PMN）抗多育赛多孢菌和阿氏赛多孢菌的抗真菌活性的影响。我们将这些活性与抗烟曲霉和黄曲霉的活性进行了比较。将PMN与IFN-γ和GM-CSF孵育22小时可增强PMN对两种曲霉属和多育赛多孢菌的菌丝损伤（p<0.05），但对阿氏赛多孢菌没有增强作用。然而，用IFN-γ和GM-CSF处理2小时后的PMN孵育后，阿氏赛多孢菌的菌丝受损明显更严重。此外，将PMN与GM-CSF孵育2小时可增强PMN的氧化爆发，以超氧阴离子（O2-）产生来衡量，这是对黄曲霉和赛多孢菌属未调理菌丝的反应（p<0.05）。相比之下，2小时后，单独的IFN-γ和GM-CSF不会增强PMN对黄曲霉和赛多孢菌属调理菌丝的O2-反应；然而，IFN-γ和GM-CSF的组合对这些菌种有显著增强作用。因此，IFN-γ和GM-CSF，特别是联合使用时，显示出PMN对赛多孢菌属反应的种属和时间依赖性增强。
