Wetscher F, Havlicek V, Huber T, Müller M, Brem G, Besenfelder U
Division of Biotechnology in Animal Production, Department for Agrobiotechnology, IFA-Tulln, BOKU-University of Natural Resources and Applied Life Sciences, Vienna, Austria.
Theriogenology. 2005 Jul 1;64(1):41-8. doi: 10.1016/j.theriogenology.2004.10.020. Epub 2004 Dec 30.
In cattle, there is no practical method, which allows tubal transfer of pre-implantation embryos for routine in vivo culture as it has been established in sheep. The aim of our study was to perform tubal transfer by transvaginal endoscopy in synchronized heifers, in order to expose embryos at various embryonic stages to the physiological mechanisms of migration in the non-ligated oviducts. Various embryonic stages were transferred by transvaginal endoscopy into the oviducts of temporary recipients and were recovered on Day 7. The transfer of embryos in hyaluronate containing medium ("Hyaluronan"), zygotes stripped of cumulus ("Denuded Zygotes"), embryos embedded in cumulus ("Zygotes with Cumulus"), matured oocytes with capacitated spermatozoa ("GIFT") or embryos embedded in Na alginate ("Alginate") led to increasing recovery rates (13, 30, 56, 63 and 71%, respectively). However, the developmental rate on Day 7 was adversely affected (16, 11, 8, 16 and 8%), whereas the blastocyst rate on Day 8 showed more balanced results (17, 14, 18, 21 and 11%). Our data demonstrate that the structural properties of transferred embryos affect tubal migration and are crucial for subsequent in vivo culture. Embryos enclosed in cumulus cells or alginate synchronize more successfully with the oviductal transport systems than denuded stages or embryos in hyaluronate containing medium.
在牛身上，目前还没有一种切实可行的方法能够像在绵羊身上那样，通过输卵管移植进行植入前胚胎的常规体内培养。我们研究的目的是通过经阴道内窥镜对同步发情的小母牛进行输卵管移植，以便使处于不同胚胎阶段的胚胎暴露于未结扎输卵管中的生理性迁移机制。通过经阴道内窥镜将不同胚胎阶段的胚胎移植到临时受体的输卵管中，并在第7天回收。将胚胎置于含透明质酸盐的培养基中（“透明质酸”）、去除卵丘的受精卵（“裸受精卵”）、包裹在卵丘中的胚胎（“带卵丘受精卵”）、带有获能精子的成熟卵母细胞（“配子输卵管内移植”）或包埋在海藻酸钠中的胚胎（“海藻酸盐”）进行移植，回收率逐渐提高（分别为13%、30%、56%、63%和71%）。然而，第7天的发育率受到不利影响（分别为16%、11%、8%、16%和8%），而第8天的囊胚率结果更为均衡（分别为17%、14%、18%、21%和11%）。我们的数据表明，移植胚胎的结构特性会影响输卵管迁移，并且对随后的体内培养至关重要。与裸胚阶段或置于含透明质酸盐培养基中的胚胎相比，包裹在卵丘细胞或海藻酸盐中的胚胎与输卵管运输系统的同步性更好。
