Schnettler Reinhard, Pfefferle Hans-Joachim, Kilian Olaf, Heiss Christian, Kreuter Jörg, Lommel Dirk, Pavlidis Theodoros, Stahl Jens-Peter, Meyer Christof, Wenisch Sabine, Alt Volker
Department of Trauma Surgery, University Hospital Giessen, Rudolf-Buchheim-Str. 7, 35385 Giessen, Germany.
J Control Release. 2005 Aug 18;106(1-2):154-61. doi: 10.1016/j.jconrel.2005.04.011.
Glycerol-l-lactide as coating polymer for the delivery of basic fibroblast growth factor (bFGF) from hydroxyapatite (HA) ceramic implants was shown to lead to significant delay in bone ingrowth into the implants compared to implants without the coating polymer. The purpose of this work was to study bone ingrowth in HA ceramic implants with and without the coating polymer but without growth factors to enable differentiation between a locking effect of the pores by the polymer and the fact of inactivation of the growth factors by the polymer, which could both be possible for the delay. A defect was created in the subchondral region of both femurs in 24 miniature-pigs and was either filled by the HA implants with or without the coating polymer. Histomorphometry showed a significant delay in bone ingrowth in the polymer coated implants both after 6 and 12 weeks. Detailed histology revealed that the HA pores were completely "locked" by the polymer leading to complete loss of the osteoconductive properties of the HA. Also electron microscopy showed filling of the HA pores by the polymer. Therefore, it can be concluded that glycerol-l-lactide should not be used to coat HA ceramic implants due to significant delay in bone ingrowth.
与未涂覆聚合物的植入物相比，甘油 - L - 丙交酯作为从羟基磷灰石（HA）陶瓷植入物中递送碱性成纤维细胞生长因子（bFGF）的涂层聚合物，已被证明会显著延迟骨长入植入物。这项工作的目的是研究有涂层聚合物和无涂层聚合物但无生长因子的HA陶瓷植入物中的骨长入情况，以便区分聚合物对孔隙的封闭作用和聚合物使生长因子失活的情况，这两种情况都可能导致延迟。在24只小型猪的双侧股骨软骨下区域制造缺损，并用有或无涂层聚合物的HA植入物填充。组织形态计量学显示，在6周和12周后，聚合物涂层植入物中的骨长入均显著延迟。详细的组织学检查显示，HA孔隙被聚合物完全“封闭”，导致HA的骨传导性能完全丧失。电子显微镜检查也显示聚合物填充了HA孔隙。因此，可以得出结论，由于骨长入显著延迟，甘油 - L - 丙交酯不应被用于涂覆HA陶瓷植入物。
