McComb Chloe, Rosenegger David, Varshney Nishi, Kwok Hiu Yee, Lukowiak Ken
Department of Physiology and Biophysics, Neuroscience Research Group, University of Calgary, 3330 Hospital Drive NW, Calgary, Alta., Canada T2N 4N1.
Neurobiol Learn Mem. 2005 Jul;84(1):9-24. doi: 10.1016/j.nlm.2005.02.002.
Operant conditioning of aerial respiratory behaviour and its consolidation into long-term memory in Lymnaea has been previously studied in both intact, freely moving snails and in in vitro preparations made from previously trained snails. Here, we show in previously untrained semi-intact in vitro Lymnaea preparations that aerial respiratory behaviour can also be operantly conditioned. Neither yoked control nor 'run-down' control procedures in these in vitro preparations result in an alteration of aerial respiratory behaviour. Memory in the operantly trained semi-intact preparations persists for at least 1h after training. Intracellular recordings made from RPeD1, one of the 3-CPG neurons and the neuron that initiates CPG activity; show that there are specific changes in central excitatory input to this neuron concurrent with learning and its consolidation into memory. In addition following the acquisition of learning and its consolidation into memory the ability of RPeD1 and VI/J neurons when depolarized to cause a pneumostome opening is significantly decreased. Thus, previously untrained in vitro semi-intact preparations can be used to study changes in neuronal activity in a neuron known to be both necessary for the behaviour and for memory formation.
此前，在完整、自由活动的蜗牛以及由先前训练过的蜗牛制成的体外制剂中，均对椎实螺空中呼吸行为的操作性条件反射及其巩固为长期记忆的过程进行过研究。在此，我们在先前未经训练的半完整体外椎实螺制剂中表明，空中呼吸行为也可进行操作性条件反射。在这些体外制剂中，无论是配对对照还是“耗尽”对照程序，均不会导致空中呼吸行为发生改变。经操作性训练的半完整制剂中的记忆在训练后至少持续1小时。从3个中枢模式发生器（CPG）神经元之一且启动CPG活动的RPeD1神经元进行的细胞内记录显示，在学习及其巩固为记忆的过程中，该神经元的中枢兴奋性输入存在特定变化。此外，在学习获得及其巩固为记忆后，RPeD1神经元和VI/J神经元去极化时引起气门张开的能力显著下降。因此，先前未经训练的体外半完整制剂可用于研究已知对行为和记忆形成均必不可少的神经元的神经元活动变化。
