Vinogradov Evgeny, Nossova Ludmila, Korenevsky Anton, Beveridge Terry J
Institute for Biological Sciences, National Research Council, 100 Sussex Dr., Ottawa, ON, Canada K1A 0R6.
Carbohydr Res. 2005 Jul 25;340(10):1750-3. doi: 10.1016/j.carres.2005.04.009.
Capsular polysaccharides were extracted from Shewanella oneidensis strain MR-4, grown on two different culture media. The polysaccharides were analyzed using 1H and 13C NMR spectroscopy, and the following structure of the repeating unit was established: [structure: see text] where the residue of 4-amino-4,6-dideoxy-D-glucose (Qui4N) was substituted with different N-acyl groups depending on the growth media. All monosaccharides are present in the pyranose form. In the PS from cells grown on enriched medium (trypticase soy broth, TSB) aerobically it was N-acylated with 3-hydroxy-3-methylbutyrate (60%) or with 3-hydroxybutyrate (40%), whereas in the PS from cells grown on minimal medium (CDM) aerobically it was acylated mostly with 3-hydroxybutyrate (>90%).
从在两种不同培养基上生长的嗜水气单胞菌MR-4菌株中提取荚膜多糖。使用1H和13C核磁共振光谱对多糖进行分析，并确定了重复单元的以下结构：[结构：见正文]，其中4-氨基-4,6-二脱氧-D-葡萄糖（Qui4N）残基根据生长培养基的不同被不同的N-酰基取代。所有单糖均以吡喃糖形式存在。在有氧条件下在富集培养基（胰蛋白酶大豆肉汤，TSB）上生长的细胞的多糖中，它被3-羟基-3-甲基丁酸（60%）或3-羟基丁酸（40%）N-酰化，而在有氧条件下在基本培养基（CDM）上生长的细胞的多糖中，它主要被3-羟基丁酸（>90%）酰化。
