Ueki Masaaki, Yamaguchi Miyuki
Department of Applied Chemistry, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan.
Carbohydr Res. 2005 Jul 25;340(10):1722-31. doi: 10.1016/j.carres.2005.04.013.
New two-component systems using quaternary ammonium or phosphonium salts as a co-matrix have been developed for the analysis of acidic carbohydrates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). In the analysis of the sodium salt of heparin disaccharide I-S, the combination of 2-amino-5-nitropyridine with tetraphenylphosphonium bromide gave the best result. In the analysis of gangliosides containing the sialic acid moiety, the combination of 2,4,6-trihydroxyacetophenone with dimethyldipalmitylammonium bromide was determined to be the system of choice. Under optimum conditions all acidic carbohydrates gave molecular ions in the form of [M(Q(n))-Q]-, where M(Q(n)) is the molecular mass of a molecule containing n molecules of quaternary ions as salt.
已开发出使用季铵盐或鏻盐作为共基质的新型双组分系统，用于通过基质辅助激光解吸/电离飞行时间质谱（MALDI-TOFMS）分析酸性碳水化合物。在肝素二糖I-S钠盐的分析中，2-氨基-5-硝基吡啶与四苯基溴化鏻的组合给出了最佳结果。在含有唾液酸部分的神经节苷脂的分析中，确定2,4,6-三羟基苯乙酮与二甲基二棕榈酰铵溴化物的组合为首选系统。在最佳条件下，所有酸性碳水化合物均以[M(Q(n))-Q]-的形式给出分子离子，其中M(Q(n))是含有n个季离子分子作为盐的分子的分子量。
