一种原核苯丙氨酸解氨酶的生化特性
Biochemical characterization of a prokaryotic phenylalanine ammonia lyase.
作者信息
Xiang Longkuan, Moore Bradley S
机构信息
College of Pharmacy, University of Arizona, Tucson, Arizona 85721-0207, USA.
出版信息
J Bacteriol. 2005 Jun;187(12):4286-9. doi: 10.1128/JB.187.12.4286-4289.2005.
Abstract
The committed biosynthetic reaction to benzoyl-coenzyme A in the marine bacterium "Streptomyces maritimus" is carried out by the novel prokaryotic phenylalanine ammonia lyase (PAL) EncP, which converts the primary amino acid L-phenylalanine to trans-cinnamic acid. Recombinant EncP is specific for L-phenylalanine and shares many biochemical features with eukaryotic PALs, which are substantially larger proteins by approximately 200 amino acid residues.
摘要
海洋细菌“海链霉菌”中对苯甲酰辅酶A的特定生物合成反应是由新型原核苯丙氨酸解氨酶（PAL）EncP进行的，它将初级氨基酸L-苯丙氨酸转化为反式肉桂酸。重组EncP对L-苯丙氨酸具有特异性，并且与真核PAL具有许多生化特征，真核PAL的蛋白质要大得多，大约多200个氨基酸残基。
相似文献
1
Biochemical characterization of a prokaryotic phenylalanine ammonia lyase.
J Bacteriol. 2005 Jun;187(12):4286-9. doi: 10.1128/JB.187.12.4286-4289.2005.
2
Inactivation, complementation, and heterologous expression of encP, a novel bacterial phenylalanine ammonia-lyase gene.
J Biol Chem. 2002 Sep 6;277(36):32505-9. doi: 10.1074/jbc.M204171200. Epub 2002 Jun 24.
3
The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases.
FEBS J. 2006 Mar;273(5):1004-19. doi: 10.1111/j.1742-4658.2006.05127.x.
4
Plant-like biosynthetic pathways in bacteria: from benzoic acid to chalcone.
J Nat Prod. 2002 Dec;65(12):1956-62. doi: 10.1021/np020230m.
5
Biochemical and Structural Analysis of Substrate Specificity of a Phenylalanine Ammonia-Lyase.
Plant Physiol. 2018 Feb;176(2):1452-1468. doi: 10.1104/pp.17.01608. Epub 2017 Dec 1.
6
Discovery of two cyanobacterial phenylalanine ammonia lyases: kinetic and structural characterization.
Biochemistry. 2007 Jan 30;46(4):1004-12. doi: 10.1021/bi061774g.
7
Kinetic analysis of the inhibition of phenylalanine ammonia-lyase by 2-aminoindan-2-phosphonic acid and other phenylalanine analogues.
Phytochemistry. 2003 Feb;62(3):415-22. doi: 10.1016/s0031-9422(02)00561-7.
8
Cinnamic acid production using Streptomyces lividans expressing phenylalanine ammonia lyase.
J Ind Microbiol Biotechnol. 2011 May;38(5):643-8. doi: 10.1007/s10295-011-0955-2. Epub 2011 Mar 22.
9
Phenylalanine ammonia lyase catalyzed synthesis of amino acids by an MIO-cofactor independent pathway.
Angew Chem Int Ed Engl. 2014 Apr 25;53(18):4652-6. doi: 10.1002/anie.201311061. Epub 2014 Apr 1.
10
Novel photoreactive cinnamic acid analogues to elucidate phenylalanine ammonia-lyase.
Bioorg Med Chem Lett. 2000 Nov 6;10(21):2481-3. doi: 10.1016/s0960-894x(00)00499-6.
引用本文的文献
1
Identification and expression analysis of genes related to chlorogenic acid synthesis in Wight.
Front Plant Sci. 2025 May 2;16:1544303. doi: 10.3389/fpls.2025.1544303. eCollection 2025.
2
Common Mitochondrial Targets of Curcumin and Cinnamic Acid, the Membrane-Active Natural Phenolic Compounds.
Pharmaceutics. 2024 Sep 29;16(10):1272. doi: 10.3390/pharmaceutics16101272.
3
Gut microbiota metabolically mediate intestinal helminth infection in zebrafish.
mSystems. 2024 Sep 17;9(9):e0054524. doi: 10.1128/msystems.00545-24. Epub 2024 Aug 27.
4
Gut microbiota metabolically mediate intestinal helminth infection in Zebrafish.
bioRxiv. 2024 Jul 27:2024.07.26.605207. doi: 10.1101/2024.07.26.605207.
5
Engineering styrene biosynthesis: designing a functional trans-cinnamic acid decarboxylase in Pseudomonas.
Microb Cell Fact. 2024 Feb 28;23(1):69. doi: 10.1186/s12934-024-02341-0.
6
Phenylpropionic acid produced by gut microbiota alleviates acetaminophen-induced hepatotoxicity.
Gut Microbes. 2023 Jan-Dec;15(1):2231590. doi: 10.1080/19490976.2023.2231590.
7
Role of dietary amino acids and microbial metabolites in the regulation of pig intestinal health.
Anim Nutr. 2021 Oct 23;9:1-6. doi: 10.1016/j.aninu.2021.10.004. eCollection 2022 Jun.
8
Biochemical Characterization of Novel Phenylalanine Ammonia-Lyase from Spirulina CPCC-695.
Protein J. 2022 Jun;41(3):414-423. doi: 10.1007/s10930-022-10063-8. Epub 2022 Jun 17.
9
Discovery of Novel Tyrosine Ammonia Lyases for the Enzymatic Synthesis of p-Coumaric Acid.
Chembiochem. 2022 May 18;23(10):e202200062. doi: 10.1002/cbic.202200062. Epub 2022 Apr 7.
10
Comparative Molecular Mechanisms of Biosynthesis of Naringenin and Related Chalcones in Actinobacteria and Plants: Relevance for the Obtention of Potent Bioactive Metabolites.
Antibiotics (Basel). 2022 Jan 10;11(1):82. doi: 10.3390/antibiotics11010082.
本文引用的文献
1
EncM, a versatile enterocin biosynthetic enzyme involved in Favorskii oxidative rearrangement, aldol condensation, and heterocycle-forming reactions.
Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15609-14. doi: 10.1073/pnas.0405508101. Epub 2004 Oct 25.
2
Crystal structure of phenylalanine ammonia lyase: multiple helix dipoles implicated in catalysis.
Biochemistry. 2004 Sep 14;43(36):11403-16. doi: 10.1021/bi049053+.
3
Context-dependent behavior of the enterocin iterative polyketide synthase; a new model for ketoreduction.
Chem Biol. 2004 Apr;11(4):461-8. doi: 10.1016/j.chembiol.2004.03.018.
4
Kinetic analysis of the 4-methylideneimidazole-5-one-containing tyrosine aminomutase in enediyne antitumor antibiotic C-1027 biosynthesis.
Biochemistry. 2003 Nov 4;42(43):12708-18. doi: 10.1021/bi035223r.
5
Mutasynthesis of enterocin and wailupemycin analogues.
J Am Chem Soc. 2003 Aug 6;125(31):9290-1. doi: 10.1021/ja035973o.
6
Investigation of the early stages in soraphen A biosynthesis.
Chem Commun (Camb). 2003 Jun 21(12):1358-9. doi: 10.1039/b303542p.
7
A novel 4-methylideneimidazole-5-one-containing tyrosine aminomutase in enediyne antitumor antibiotic C-1027 biosynthesis.
J Am Chem Soc. 2003 May 21;125(20):6062-3. doi: 10.1021/ja034609m.
8
Kinetic analysis of the inhibition of phenylalanine ammonia-lyase by 2-aminoindan-2-phosphonic acid and other phenylalanine analogues.
Phytochemistry. 2003 Feb;62(3):415-22. doi: 10.1016/s0031-9422(02)00561-7.
9
Characterization of benzoyl coenzyme A biosynthesis genes in the enterocin-producing bacterium "Streptomyces maritimus".
J Bacteriol. 2003 Jan;185(2):399-404. doi: 10.1128/JB.185.2.399-404.2003.
10
Inactivation, complementation, and heterologous expression of encP, a novel bacterial phenylalanine ammonia-lyase gene.
J Biol Chem. 2002 Sep 6;277(36):32505-9. doi: 10.1074/jbc.M204171200. Epub 2002 Jun 24.
Zotero 插件