Huang Hai-yan, Liu Jian-jun, Zhuang Zhi-xiong, Li Xi-yi, Xu Xin-yun, Wei Qin-zhi, Yang Xiao-hua
Laboratory of food and Toxicology, Shenzhen Center for Disease Control and Prevention, Shenzhen 510080, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2005 May;39(3):175-8.
To study the effects of trichloroethylene (TCE) on the protein in L-02 cells in vitro.
Thiazolyl blue and Trypan blue tests were used to investigate the cytotoxicity of TCE to L-02 liver cell. The 2-D electrophoresis was used to analyse the expression of proteins in L-02 liver cells. The differentially expressed protein spots were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-TOF-MS).
When the concentration of TCE exceeded 30 micromol/L, there was distinct cytotoxicity to L-02 cell (P < 0.05). Selected 40 micromol/L to treat L-02 liver cells and analyze the differential proteome expression, the results showed that the expression level of 37 protein spots was up-regulated and 15 protein spots was down-regulated. And 15 proteins were identified by MALDI-TOF-TOF-MS.
TCE can change the proteome expression of L-02 liver cell. It should provide the fundamental information to identify proteins related to TCE in further study.
研究三氯乙烯（TCE）对体外培养的L-02细胞蛋白质的影响。
采用噻唑蓝和台盼蓝试验研究TCE对L-02肝细胞的细胞毒性。采用二维电泳分析L-02肝细胞中蛋白质的表达情况。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-TOF-MS）鉴定差异表达的蛋白质斑点。
当TCE浓度超过30微摩尔/升时，对L-02细胞有明显的细胞毒性（P < 0.05）。选择40微摩尔/升处理L-02肝细胞并分析差异蛋白质组表达，结果显示37个蛋白质斑点的表达水平上调，15个蛋白质斑点的表达水平下调。通过MALDI-TOF-TOF-MS鉴定出15种蛋白质。
TCE可改变L-02肝细胞的蛋白质组表达。这为进一步研究鉴定与TCE相关的蛋白质提供了基础信息。
