Luo Xin-hua, Yang Qin, Zhang Quan, Cheng Ming-liang
Department of Infectious Diseases, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China.
Zhonghua Yi Xue Za Zhi. 2007 Dec 25;87(48):3411-4.
To develop the 2-DE profiles of proteome from hepatic fibrosis tissue and preliminarily analyze the differential expression of proteome.
The differential expression of proteins were analyzed by imaging analysis and MALDI-TOF-MS after the total protein was extracted from the hepatic fibrosis tissue and the normal liver tissue by 2-DE. 3 proteins were verified by in Western-blotting.
Fifty-nine differentially expressed protein were found in the proteome profile analysis of these two types of tissue, among which 30 protein spots were up-regulated and 29 protein spots were down-regulated in these hepatic fibrosis tissues. Fifteen protein spots with the differential expressions more than 3 times were analyzed by peptide mass fingerprint (PMF) and 10 differentially expressed proteins were found to be related with cell signal transduction, cell proliferation, and oxidative stress. Western-blotting results of 14-3-3beta, DJ-1, and PEBP were consistent with those by the 2-DE examination.
Some differentially expressed proteins have been found in the hepatic fibrosis tissue and the normal liver tissue. These data provided a fundamental basis for further study of the mechanism of hepatic fibrogenesis. Proteome;
构建肝纤维化组织蛋白质组的双向电泳图谱，并初步分析蛋白质组的差异表达。
通过双向电泳从肝纤维化组织和正常肝组织中提取总蛋白后，采用图像分析和基质辅助激光解吸电离飞行时间质谱分析蛋白质的差异表达。通过蛋白质免疫印迹法验证3种蛋白质。
在这两种组织的蛋白质组图谱分析中发现59种差异表达蛋白，其中肝纤维化组织中有30个蛋白点上调，29个蛋白点下调。对差异表达倍数超过3倍的15个蛋白点进行肽质量指纹图谱分析，发现10种差异表达蛋白与细胞信号转导、细胞增殖和氧化应激有关。14-3-3β、DJ-1和磷酸烯醇式丙酮酸羧激酶的蛋白质免疫印迹结果与双向电泳检测结果一致。
在肝纤维化组织和正常肝组织中发现了一些差异表达蛋白。这些数据为进一步研究肝纤维化发生机制提供了基础。蛋白质组；
