[Differential analysis of associated proteome in hepatic fibrosis tissue].

OBJECTIVE

To develop the 2-DE profiles of proteome from hepatic fibrosis tissue and preliminarily analyze the differential expression of proteome.

METHODS

The differential expression of proteins were analyzed by imaging analysis and MALDI-TOF-MS after the total protein was extracted from the hepatic fibrosis tissue and the normal liver tissue by 2-DE. 3 proteins were verified by in Western-blotting.

RESULTS

Fifty-nine differentially expressed protein were found in the proteome profile analysis of these two types of tissue, among which 30 protein spots were up-regulated and 29 protein spots were down-regulated in these hepatic fibrosis tissues. Fifteen protein spots with the differential expressions more than 3 times were analyzed by peptide mass fingerprint (PMF) and 10 differentially expressed proteins were found to be related with cell signal transduction, cell proliferation, and oxidative stress. Western-blotting results of 14-3-3beta, DJ-1, and PEBP were consistent with those by the 2-DE examination.

CONCLUSION

Some differentially expressed proteins have been found in the hepatic fibrosis tissue and the normal liver tissue. These data provided a fundamental basis for further study of the mechanism of hepatic fibrogenesis. Proteome;